槲皮素对兔离体肠平滑肌张力的影响及其机制  

Influences of quercetin on contraction of small intestinal smooth muscle of rabbits in vitro and its mechanism of action

作　　者：唐学敏[1] 李家栋[1] 杨静[2] 王巧云[2] 

机构地区：[1]滨州医学院口腔学院,山东烟台264000 [2]滨州医学院基础学院,山东烟台264000

出　　处：《中国医院药学杂志》2013年第3期198-202,共5页Chinese Journal of Hospital Pharmacy

基　　金：山东省高等学校优秀青年教师国内访问学者项目(编号:2009GN003);2011年滨州医学院大学生科技创新项目(编号:BY2011DKCX12)

摘　　要：目的:观察槲皮素对兔离体回肠平滑肌肌张力的影响,并探讨其作用机制。方法:试验采用经典的离体小肠灌流技术,观察槲皮素(quercetin,Que)对回肠平滑肌自主收缩活动以及乙酰胆碱(acetylcholine,Ach)、氯化钡及组胺(histamine)所致痉挛性收缩肠平滑肌张力的影响。结果:槲皮素(20,60,100mol.L-1)能剂量依赖性的抑制家兔离体小肠平滑肌自发性收缩,对乙酰胆碱,组胺和氯化钡所致的兔离体肠痉挛性收缩也具有剂量依赖性抑制作用。在无钙台式液中,槲皮素可明显抑制Ach引起的回肠平滑肌收缩(P<0.01),亦能使氯化钙量效曲线下移。槲皮素对Bay K8644(0.5mol.L-1)引起的回肠平滑肌收缩亦有明显抑制作用(P<0.01)。结论:槲皮素明显抑制了家兔离体小肠平滑肌的收缩活动,其机制与抑制外钙内流及内钙释放有关。OBJECTIVE To observe the influences of quercetin on the contraction of small intestine smooth muscle of rabbits in vitro and explore the mechanism of action. METHODS With the isothermal perfusion of small intestine in vitro, the relaxant effects and related mechanism of quercetin were studied on the spontaneous contraction of small intestinal and contraction induced by Ach, histamine and Bacl2. RESULTS Quercetin reduced the actonmic contractivity of small intestine smooth muscle antonomic and the cmtractinity induced by acetylcholine. BaC12 and histamine （P〈0. 01 ）. Quercetin were found to inhibit the conlraction induced bv acetychline in the Ca2＋ free Tyrode＇ s solution and the CaCl2 cumulative dose-response curves with a lower maximal response. And it could also depress the contraction agonized by Bay K8644 in small intestinal smooth muscle. CONCLUSION Quercetin inhibits the contraction of small intestinal smooth muscle of rabbits in vitro. The mechanism may be related to inhibit the extracellular Ca2＋ inflowing via cell membrane channel and intracellular Ca2＋ releasing via sarcoplasmic reticulum.

关 键 词：槲皮素 小肠平滑肌 钙离子 

分 类 号：R963[医药卫生—微生物与生化药学]