筋骨草总黄酮的HPD300大孔树脂动态吸附纯化工艺探讨  

Purification of total flavonoids from Ajuga decumbens by HPD300 macroporous resin

作　　者：施淑琴[1] 施群[2] 

机构地区：[1]金华职业技术学院医学院,浙江金华321007 [2]金华市中心医院,浙江金华321000

出　　处：《中国医院药学杂志》2013年第3期212-214,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的:确定HPD300大孔吸附树脂吸附纯化筋骨草总黄酮的工艺条件。方法:采用动态吸附分离法,确定筋骨草总黄酮的纯化工艺条件。结果:HPD300大孔树脂对筋骨草总黄酮有良好的吸附分离性能,其动态吸附分离工艺条件为:筋骨草总黄酮上样质量浓度为4mg.mL-1,最大吸附量为13.73mg.g-1,吸附流速为1mL.min-1,以10倍柱体积70%乙醇洗脱,树脂可重复使用4次。结论:采用HPD300大孔树脂吸附分离筋骨草总黄酮简便有效,回收率高。OBJECTIVE To investigate the technological conditions of purification of total flavonoids extracted from Ajuga decumbens by HPD300 macroporous resin. METHODS The dynamic conditions of adsorption and separation were analyed with HPD300 macroporous resin. The flavonoids adsorption capacity was used as the evaluation criteria. RESULIS HPD300 had the good performance for adsorbing and separating total flavonoids from Pu decumbens in the following technological conditions： the concentration of the flavonoids sample extract was 5 mg·mL- 1 ; the maximum adsorbing capacity was 13. 73 mg·g-1; the current velocity was I mL.min-1; the elutingreagent was 50% ethanol ten times as the volume of the resin; and HPD300 resin could be used four times, repeatedly. CONCLUSION The method of adopting HPD300 maeroporous resin to adsorb and separate total flavonoids from A. decumbens is simple and efficient and the total flavone recovery rate is high.

关 键 词：筋骨草 总黄酮 大孔树脂 吸附纯化 

分 类 号：R95[医药卫生—药学]