14-3-3蛋白调节1-细胞期小鼠受精卵有丝分裂  

Regulation of 14-3-3 in the First Mitotic Cell Cycle in One-cell Stage Mouse Fertilized Eggs

作　　者：崔城[1] 秦鑫[1] 任秀丽[1] 于秉治[2] 

机构地区：[1]中国医科大学基础医学院生理学教研室,沈阳110001 [2]中国医科大学基础医学院生物化学与分子生物学教研室,沈阳110001

出　　处：《中国医科大学学报》2013年第1期19-22,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30900514);辽宁省教育厅高校科研计划(2008S224)

摘　　要：目的研究14-3-3蛋白在1-细胞期小鼠受精卵有丝分裂中的作用。方法 RT-PCR技术鉴定小鼠受精卵14-3-3蛋白亚型。采用显微注射方法将14-3-3 siRNA注射入小鼠受精卵G1期,观察受精卵的卵裂率、形态学变化及MPF活性。结果小鼠受精卵中的14-3-3蛋白亚型是14-3-3ε。小鼠受精卵注射pSUPER-14-3-3εsiRNA后,与对照组相比,卵裂率下降,有丝分裂延迟,有更多的受精卵发生形态异常,MPF活性最高值显著下降。结论 14-3-3蛋白在调节小鼠受精卵有丝分裂中发挥重要作用。Objective To study the effects of 14-3-3 proteins in regulation of the first mitotic cell cycle in one-cell stage mouse fertilized eggs. Methods 14-3-3 isoform in the mouse fertilized eggs was identified by RT-PCR. 14-3-3 siRNA was introduced to G1 phase fertilized eggs by microinjection to study the cleavage rate, morphology and MPF activity. Results 14-3-3 e was identified in one-cell stage of mouse fertilized eggs. Compared with the control group,the cleavage rate in pSUPER-14-3-3e siRNA injection group was significantly decreased, mitosis was delayed and more abnormal morphology eggs were observed. Moreover, the maximal value of MPF activity was significantly de- creased. Conclusion 14-3-3 proteins play critical roles in the first mitotic cell cycle in mouse fertilized eggs.

关 键 词：14—3—3蛋白 1-细胞期受精卵 细胞周期 

分 类 号：R34[医药卫生—基础医学]