樟树优良家系的组培育苗技术研究  

Tissue Culture and Micropropagation of Superior Families of Cinnamomum camphora(L.)Presl.

作　　者：周丽华[1] 蔡燕灵[1] 曾令海[1] 蔡静如[1] 张谦[1] 

机构地区：[1]广东省林业科学研究院,广东广州510520

出　　处：《热带作物学报》2013年第1期67-73,共7页Chinese Journal of Tropical Crops

基　　金：北京林业大学林木育种国家工程实验室开放课题项目(No.FOP2010-12);广东省林业科技创新专项(No.2008/09/10/11KJCX001-01);中央财政林业科技推广示范项目(No.[2011]GDTK-01);林业公益性行业科研专项经费子项目(No.20110400103)

摘　　要：以新选育的珍贵用材树种樟树的2个优良家系为材料,开展组培快繁育苗技术研究。结果表明,樟树适宜初代培养基为改良DCR(DCRI)附加6-BA 0.3 mg/L和NAA 0.05 mg/L。家系X5和X9的最佳继代培养基分别为DCRI+6-BA 0.6 mg/L+NAA 0.05 mg/L和DCRI+6-BA 1.0 mg/L+IAA 0.2 mg/L+NAA 0.05 mg/L,其芽倍数分别为增殖2.69倍和3.25倍。通过优化组合,筛选得到2个家系的通用生根培养基:1/2MS+IBA 2.0 mg/L+IAA 1.7 mg/L+6-BA 0.1 mg/L+NAA 0.05 mg/L,其生根率高达96.30%。探索出适宜的驯化、移栽和后期管理技术,使2个家系生根苗的移栽存活率分别高达85.2%和89.7%。Two newly-selected superior families of Cinnamomum camphora were used for tissue culture and micropropagation. C. camphora explants displayed the optimum initial growth on the primary media, including improved basic DCR（DCR1）supplemented with 6-BA 0.3 mg/L and NAA 0.05 mg/L. Family X5 showed the highest shoot proliferation rate of 2.69 times on subculture DCRI＋6-BA 0.6 mg/L＋NAA 0.05 mg/L and family X9 exhibited the highest rate of 3.25 times on subculture DCRI＋6-BA 1.0 mg/L＋IAA 0.2 mg/L＋NAA 0.05 mg/L. An optimization experiment revealed one common rooting medium： 1/2MS＋IBA 2.0 mg/L＋IAA 1.7 mg/L＋6-BA 0.1 mg/L＋NAA 0.05 mg/L, for two families. The rooting rate was 96.30%. Suitable techniques and procedures were identified for acclimatization, transplantation and later-period management of tissue-cultured plantlets. The survival rates of two families were 85.2% and 89.7%, respectively.

关 键 词：樟树 优良家系 组织培养 

分 类 号：S792.23[农业科学—林木遗传育种] S603[农业科学—林学]