大蕉枯萎病病原菌的分离、鉴定和致病性测定  

Isolation and Identification of Plantain(Musa paradisiaca Linn.) Fusarium Wilt Disease Pathogen and Pathogenicity Test

作　　者：郭立佳[1] 彭军[1] 杨腊英[1] 王国芬[1] 梁昌聪[1] 刘磊[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室,海南海口571101

出　　处：《热带作物学报》2013年第1期105-110,共6页Chinese Journal of Tropical Crops

基　　金：国家公益性行业(农业)科研专项"由尖孢镰刀菌引起的作物土传病害研究"(No.200903049)

摘　　要：从表现枯萎病症状的大蕉球茎病组织中获得10株分离物,采用形态学分类方法将这些分离物鉴定为尖孢镰刀菌。利用分离物DJ1的rDNA-ITS区序列和IGS序列开展的系统发育分析进一步确定DJ1为尖孢镰刀菌。对不同香蕉品系的致病性测定结果表明,DJ1对粉蕉(Musa sp.ABB)的致病性最强,其次是特威(Musa sp. AAA)、巴西蕉(Musa sp.AAA)和泰蕉(Musa sp.AAA),对皇帝蕉(Musa sp.AA)的致病性较弱,根据DJ1的寄主范围确定DJ1为尖孢镰刀菌古巴专化型生理4号小种。对IGS序列的进一步比对分析表明DJ1不属于热带4号生理小种菌株。这些结果为大蕉枯萎病的防治提供了重要依据和指导。Plantain （Musa paradisiaca Linn.） is an edible banana, and the causal agent of plantain wilt disease hasn＇t been reported until now. In this study, ten isolates （DJI-10） from the corms of the plantains that showed wih symptoms were isolated. These isolates were preliminarily identified as Fusarium oxysporm based on their morphological characteristics. Then phylogenetic analysis based on the sequences of rDNA-ITS and intergenic spacer （IGS） regions added proof that the isolate DJ1 was F. oxysporum. The pathogenicity assays showed that DJ1 was able to infect 5 varieties of the genus Musa including Fenjiao （Musa sp. ABB）, Hangdijiao （Musa sp. AA）, Taijiao （Musa sp. AAA）, Tewei （Musa sp. AAA） and Brazilian （Musa sp. AAA）, but it was most pathogenic to Fenjiao, then Tewei, Taijiao and Bazilian, and it was less pathogenic to Huangdijiao. These results imply that DJ1 might be an isolate from race 4 of Fusarium oxysporum f. sp. cubense （Foc）.

关 键 词：大蕉 枯萎病 系统发育分析 致病性 尖孢镰刀菌 

分 类 号：S432.4[农业科学—植物病理学]