激素抵抗性哮喘患者外周血CD4^＋CD25^＋Foxp^3＋调节性T细胞及IL-10、TGF-β1的变化及意义  

Changes and clinical significance of CD4^＋ CD25^＋ Foxp3^＋ regulatory T cell and IL-10, TGF-β1 in steroid- resistant asthma patients of peripheral blood

作　　者：钱一龙[1] 赵振中[1] 朱建俊[1] 谢中华[1] 王珠美[1] 

机构地区：[1]杭州市余杭区第二人民医院内二科,311121

出　　处：《国际呼吸杂志》2013年第3期161-164,共4页International Journal of Respiration

摘　　要：目的观察激素抵抗性哮喘（SRA）患者外周血CD4^＋CD25^＋Foxp^3＋调节性T细胞（Treg）及白介素10（IL-10）、转化生长因子β-（TGF—β1）的变化，分析其在SRA发病机制中的作用。方法采用流式细胞术检测40例SRA患者（激素抵抗组）外周血单个核细胞CD4^＋CD25^＋Foxp^3＋Treg数目，并计算CD4^＋CD25^＋Foxp^3＋Treg占CD4^＋T淋巴细胞的百分比；酶联免疫吸附试验（ELISA）法检测其血清IL-10、TGF-β1水平，并与激素敏感性患者（激素敏感组，46例）及正常体检者（正常组，30例）进行对比。结果激素抵抗组患者外周血CD4^＋CD25^＋Foxp^3＋Treg占CD4^＋T淋巴细胞的百分比、CD4^＋CD25^＋Foxp^3＋Treg绝对值及血清IL-10、TGF—β1水平均明显低于激素敏感组与正常组（P〈0．01，P〈0．05）；激素敏感组患者外周血CD4^＋CD25^＋Foxp^3＋Treg占CD4^＋T淋巴细胞的百分比、CD4^＋CD25^＋Foxp^3＋Treg绝对值及血清TGF-β1水平明显低于正常组（P〈0．01，P〈0．05），血清IL-10无明显差异（P〉0．05）；CD4^＋CD25^＋Foxp^3＋Treg／CD4^＋T及CD4^＋CD25^＋Foxp^3＋Treg绝对数均与血清IL-10、TGF-β1水平呈明显正相关（P〈0．01）。结论SRA患者外周血CD4^＋CD25^＋Foxp^3＋Treg数目减少及IL-10、TGF-β1含量减低可能与SRA的发生、发展有关。Objective To investigate the changes of CD4^＋ CD25^＋ Foxp3^＋ regulatory T cell and IL-10, TGF-β1 in steroid resistant asthma patients of peripheral blood, to study their significance. Methods The CD4^＋ CD25^＋ Foxp3^＋ regulatory T cell in 40 cases steroid-resistant asthma patients were detected by flow eytometry. The levels of serum IL-10 and TGF-β1 were detected by ELISA. The control groups were 30 individuals having general physical examination and 46 patients with steroid-sensitive patients. Results The proportion and number of CD4^＋ CD25^＋ Foxp3^＋ regulatory T cell and the levels of serum 1L-10,TGF-β1 in steroid-resistant asthma group of Peripheral Blood were decreased significantly than steroid-sensitive group and healthy control group （ P 〈0.01, P 〈0.05）. The proportion and number of CD4^＋ CD25^＋ Foxp3^＋ regulatory T cell and the levels of serum TGF-β1 in steroid-sensitive group were decreased significantly than ,healthy control group, but the levels of serum IL-10 were no statistical difference between them. The proportion and number of CD4^＋ CD25^＋ Foxp3^＋ regulatory T cell were positively correlated with the serum IL 10,TGF-β1 （ P 〈0.01）. Conclusions Lower proportion and number of CD4^＋ CD25^＋ Foxp3^＋ regulatory T cell and lower IL-10,TGF-β1 were observed in steroid-resistant asthma patients, which might play an important role in occurrence and development of steroid-resistant asthma.

关 键 词：激素抵抗性哮喘 调节性T细胞 转化生长因子β1 白介素-10 

分 类 号：R562.25[医药卫生—呼吸系统]