南海沉积环境来源真菌Eurotium sp.SCSIO F452的次生代谢产物研究  

Analysis of Secondary metabolites produced by Eurotium sp.SCSIO F452 isolated from the South China Sea Sediment

作　　者：王发左[1] 黄智[1] 史雪凤[1] 陈玉婵[2] 田新朋[1] 李洁[1] 章卫民[2] 张偲[1] 

机构地区：[1]中国科学院南海海洋研究所 中国科学院海洋生物资源可持续利用重点实验室 中国科学院海洋微生物研究中心 广东省海洋海洋药物重点实验室,广东广州510301 [2]广东省微生物研究所,广东广州510070

出　　处：《中国海洋药物》2013年第1期7-12,共6页Chinese Journal of Marine Drugs

基　　金：国家重点基础研究发展计划项目(2010CB833800);国家高技术研究发展计划(2012AA092104);国家自然科学青年基金(40906076;40906075)资助

摘　　要：目的对1株南海沉积环境来源真菌的次生代谢产物进行分离、鉴定及活性研究。方法采用溶剂萃取、硅胶柱层析、凝胶柱层析等方法对真菌Eurotiumsp.SCSIO F452的发酵产物进行化学分离,通过NMR、MS等波谱学技术并参阅文献进行化合物结构鉴定,采用SRB法评价化合物的细胞毒活性。结果从菌株SCSIO F452中分离鉴定6个单体化合物,分别为:isodihydroauroglaucin(1)、flavoglaucin(2)、tetrahydroauro-glaucin(3)、2-(1,1-dimethyl-2-propen-1-yl)-1H-indole-3-carboxaldehyde(4)、neoechinulin A(5)和methyl lino-leate(6)。化合物1-5对4种肿瘤细胞系表现出不同强度的细胞毒活性。结论苯甲醛衍生物1-3是真菌SC-SIO F452的优势代谢产物,细胞毒活性较强,具有潜在的研究价值。Objective To isolate and identify the secondary metabolites of Eurotium sp. SCSIO F452 isola ted from the South China Sea sediment. Methods The fermentation products were purified by solvent extraction and column chromatography （silica gel and sephadex LH-20）. The isolated compounds were identified by spectroscopic analysis （NMR and MS） as well as comparison with literatures. Their cyto toxicities were evaluated by SRB method. Results Six compounds were isolated from the acetic ether ex tracts of strain SCSIO F452, and their structures were determined as isodihydroauroglaucin（1）, fla voglaucin（2）, tetrahydroauroglaucin （3）, 2-（1,1-dimethyl 2-propen-l-yl）-lH indole-3-carboxaldehyde （4）, neoechinulin A（5）, and methyl linoleate（6）. Compounds 1-5 showed varied cytotoxic activities a gainst four cancer cell lines. Conclusion Benzaldehyde derivatives 1 3 were dominant metabolites of strain SCSIO F452 and exhibited moderate cytotoxicities. These compounds showed strong potentials for further research.

关 键 词：海洋真菌 散囊菌 次生代谢产物 细胞毒活性 沉积物 

分 类 号：R915[医药卫生—微生物与生化药学] O629[医药卫生—药学]