柑桔黄龙病LAMP快速检测方法的建立  

Development and Evaluation of A Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Huanglongbing

作　　者：孔德英[1] 孙涛[1] 李应国[1] 滕少娜[1] 陆丽华[1] 邓朝晖[1] 

机构地区：[1]重庆出入境检验检疫局,重庆400020

出　　处：《中国南方果树》2013年第1期8-11,共4页South China Fruits

基　　金：国家质检总局科研项目(2011IK186)资助

摘　　要：根据GenBank中柑桔黄龙病菌的外膜蛋白(OMP)基因序列,设计了一套环介导等温扩增引物,建立了柑桔黄龙病的环介导等温扩增检测方法。该方法灵敏度可达25pg/μL,与实时荧光PCR方法灵敏度相同。全部反应只需一个可控温的水浴锅在60分钟内即可完成,通过肉眼观察浊度状况即可判定结果。该方法具有简便、快速、灵敏、特异等特点,适用于基层工作站的柑桔黄龙病鉴定工作。Based on the conserved sequences of the outer membrane protein（OMP） gene of Candidatus Liberobacter asiaticus from GenBank,a set of the loop-mediated isothermal amplification（LAMP） primers was designed to amplify the target genes.The LAMP assay was established to detect HLB.The results showed that the detection limit of HLB-LAMP was 25 pg/μL,same as the real-time PCR method.The amplification could be conducted with a water bath within 60mins.The result could be visualized by naked eyes.This HLB-LAMP method is simple,rapid,sensitive,specific and suitable for primary workstation to detect HLB.

关 键 词：柑桔黄龙病 环介导等温扩增 检测 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]