糖肾宁对早期糖尿病肾病大鼠肾组织非酶糖基化终产物的影响  

Effect of Tangshenning on Nonenzymatic Advanced Glycation End Products of Nephridial Tissue in Early Diabetic Nephropathy Rats

作　　者：王东[1] 吴同茹[1] 谢婷婷[1] 黄迪[1,2,3] 陈刚[1,2,3] 曹和欣[1,2,3] 何立群[1,2,3] 

机构地区：[1]上海中医药大学附属曙光医院,上海200021 [2]上海市中医临床重点实验室,上海200021 [3]上海市高校中医内科E-研究院,上海200021

出　　处：《四川中医》2013年第1期53-55,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：浦东新区中医中青年骨干培养项目(PWZ2008-20-q02);上海市科学技术委员会科研计划项目(11ZR1437700);上海市教育委员会科研创新项目(09JW32);上海市卫生局中医药科研基金(2008L046A);上海市教育委员会E-研究院建设计划资助项目(E03008);上海高校创新团队建设项目

摘　　要：目的:探讨糖肾宁对早期糖尿病肾病(DN)大鼠肾组织非酶糖基化终产物(AGEs)的影响。方法:雄性SD大鼠32只,随机取8只为正常对照组(A组),给予普通饲料喂养,4周后,腹腔注射枸橼酸-枸橼酸钠缓冲液(30mg/Kg);其余24只为糖尿病(DM)造模组,采用高脂饲料喂养,4周后,按30mg/Kg腹腔注射链脲佐菌素(STZ)建立DM大鼠模型,2周后,测定24h尿微量白蛋白排泄量(24hU-A1b),按24hU-A1b排泄量随机分为模型组(B组)、糖肾宁组(C组)、氨基胍组(D组)。8周后,检测各组大鼠血糖、24hU-Alb以及肾组织AGEs的水平。结果:B组大鼠血糖、24hU-Alb、AGEs水平明显高于A组(均P<0.01)。经糖肾宁、氨基胍治疗后,24hU-Alb、AGEs水平均有所下降,与B组大鼠差别有统计学意义(均P<0.01),但仍高于A组(均P<0.05);同时,经糖肾宁治疗后大鼠血糖明显下降,而D组大鼠血糖无明显变化。结论:糖肾宁具有氨基胍类似作用,可抑制蛋白质非酶糖基化反应,并具有降低血糖、24hU-Alb的作用。To study the effect of Tangshenning on nonenzymatic advanced glycation end products（AGEs） of nephridial tissue in early diabetic nephropathy rats.Methods：32 male SD rats were randomly divided into normal control group（group A,n=8） and diabetic group（n=24）.Group A with conventional diet for 4 weeks,was injected intraperitoneally with 30mg/kg of citrate buffer.Diabetic group with high fat diet for 4 weeks,was injected intraperitoneally with 30mg/kg of STZ.After 2 weeks,24hU-Alb was measured.According to 24hU-Alb,the 24 SD rats were randomly divided into model group（group B,n=8）,Tangshenning group（group C,n=8） and aminoguanidine group（group D,n=8）.After 8 weeks,the level of blood glucose,24hU-Alb and tissue AGEs were measured.Results：The levels of blood glucose,24hU-Alb and AGEs in rats of group B were obviously higher than those of group A（all P0.01）.The levels of 24hU-Alb and AGEs of group C and D were obviously lower than those of group B（all P0.01）,but higher than those of group A（all P0.05）.The levels of blood glucose of group C was obviously lower than those of group B,but group D was not obviously change.Conclusion：Similar to aminoguanidine,Tangshenning has effect on inhibiting the action of proteinum non-enzymatic glycosylation reaction and decreasing blood glucose and 24hU-Alb.

关 键 词：糖肾宁 氨基胍 糖尿病肾病 糖基化终产物 

分 类 号：R285.5[医药卫生—中药学]