红花檵木CHS基因的克隆与序列分析  

Cloning and Sequence Analyzing of Chalcone Synthase Gene in Loropetalum chinense var.rubrum

作　　者：许威[1] 于晓英[1] 陈己任[1] 符红艳[1] 胡博文[1] 陈彦斌[1] 李达[1] 

机构地区：[1]湖南农业大学园艺园林学院观赏园艺系,长沙410128

出　　处：《中国农学通报》2013年第1期24-28,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然基金面上项目"DREB和XET基因聚合表达的分子基础"(31071826);湖南省高等学校科学研究项目重点项目"红花檵木叶色高温返青的分子机理研究"(10A057)

摘　　要：查尔酮合酶(chalcone synthase,CHS)是进入类黄酮和花色素苷次生代谢的第1个关键酶。根据植物查尔酮合成酶保守区序列设计引物,以红花檵木(Loropetalurn chinense var.rubrum)大叶红的嫩叶为材料,用RT-PCR方法,分离得到了一个查尔酮合成酶基因的cDNA(GenBank登录号为JQ609678),将该基因命名为LcvrCHS1。该序列长927bp,编码232个氨基酸残基。其核苷酸序列与GenBank已登录的同样来源的核桃、山茶属植物CHS序列同源性达83%,与其他科植物(绣球花、葡萄、桃、马铃薯、甘草、领春木属)CHS序列同源性也达到80%以上;其编码的氨基酸序列与山茶属、葡萄、鳄梨、洋梨、沙梨、映山红CHS基因编码的氨基酸序列同样具有高度同源性,同源性高达98%。Chalcone synthase（chalcone synthase,CHS） is the key enzyme that catalyzes the first step in flavonoids biosynthesis and anthocyanins secondary metabolites.A full-length cDNA encoding CHS was cloned from the young leaves of Loropetalurn chinense var.rubrum by RT-PCR using specific primers based on the highly conserved sequences of plant CHS that had already known.Blast search revealed that it was a new gene,and was named as LcvrCHS1（GenBank accession：JQ609678）.The sequence was 927 bp,encoding 232 amino acid residues.It had 83% sequence homology with walnut and camellia that had been logged in GenBank;with other genus plants（hydrangea,grapes,peaches,potatoes,licorice,Euptelea genus）,CHS sequence homology was also more than 80%;with other plants（camellia,grapes,avocados,bartlett pear,sand pear,azalea）,CHS sequence also had high homology,up to 98% homology.

关 键 词：红花檵木 查尔酮合成酶基因 克隆 序列分析 

分 类 号：S687.2[农业科学—观赏园艺]