乙烯利浸种对聚乙二醇胁迫下玉米种子萌发的影响  

The Effects of Ethephon on the Germination of Corn Seeds Stressed by Polyethylene Glycol

作　　者：闫秋洁[1] 刘再婕[1] 杨欣[1] 

机构地区：[1]绵阳师范学院生命科学与技术学院遗传与细胞学实验室,四川绵阳621000

出　　处：《中国农学通报》2013年第3期53-58,共6页Chinese Agricultural Science Bulletin

基　　金：四川省教育厅基金"重金属离子和叠氮化钠对蚕豆和玉米的影响研究"(10ZB140)

摘　　要：为了优选出能使玉米种子在干旱胁迫下最佳萌发的乙烯利浸种浓度,用0、100、200、400、800mg/L的乙烯利对玉米(‘三北2号’)种子浸种24h,再分别用0%、5%、10%、15%的聚乙二醇(PEG6000)模拟干旱处理,测定20个双重胁迫处理组合下玉米种子发芽率、幼苗长度、MDA含量、CAT活性和POD活性,并运用方差分析和多重比较方法进行差异显著性检验。结果表明:(1)100～800mg/L的乙烯利都能提高PEG6000胁迫下玉米种子的发芽率和幼苗长度,同时降低MDA含量,增强POD和CAT活性。(2)各指标在200mg/L乙烯利浸种时与相同PEG水平下0mg/L乙烯利处理有显著差异(P<0.05)。200mg/L乙烯利浸种能显著缓解玉米幼苗在5%～15%PEG6000模拟下的干旱胁迫。To explore the optimization concentration of ethephon on the germination of corn seeds under drought stress,corn seeds were soaked by ethephon with the concentrations of 0 mg/L,100 mg/L,200 mg/L,400 mg/L and 800 mg/L respectively for 24 hours.After that,the corn seeds were treated with PEG6000 for 7 days respectively for the simulation of different drought stress.The concentrations of PEG6000 were 0%,5%,10% and 15% respectively.And then,the germination rate,seedling length,MDA content,CAT activity and POD activity of the corn seedling under the double stress of every treatment were determined.And the variance analysis and multiple comparisons were used to study the relationship of different treatments.The results showed that（1） the germination rate,seedling length;CAT activity and POD activity of the corn were enhanced with the ethephon of 100-800 mg/L under the same PEG6000 drought stress,and the seedling MDA content were reduced with it.（2）There were significant differences（P0.05） between 200 mg/L ethephon treatment and 0 mg/L ethephon treatment at the same PEG6000 concentration on each determined index.Therefore,200 mg/L ethephon could alleviate the drought stress of the corn seedling simulated by PEG6000 with the concentrations of 5%-15%.

关 键 词：乙烯利 浸种 聚乙二醇 玉米 

分 类 号：S332.4[农业科学—作物遗传育种]