运动对胰岛素抵抗大鼠瘦素及其受体表达的影响  

Effect of Exercise on Expression of Leptin and Its Receptor in Rats with Insulin Resistance

作　　者：宁采亭[1] 蔡颖[1] 刘遂心[1] 谢康玲[1] 张文亮[1] 刘元[1] 

机构地区：[1]中南大学湘雅医院心血管病康复中心,湖南长沙市410008

出　　处：《中国康复理论与实践》2013年第1期46-49,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：湖南省科学技术厅科技计划项目(06FJ4096);湖南省卫生厅科研基金项目(B2006-036)

摘　　要：目的观察运动对胰岛素抵抗大鼠血清瘦素水平及组织瘦素受体表达的影响。方法 9只SD大鼠普通饲料喂养作为对照组。将胰岛素抵抗造模成功的34只大鼠随机分为4组:模型组9只:高脂高糖喂养;二甲双胍组8只:高脂高糖喂养加用二甲双胍300 mg/kg.d;运动组9只:高脂高糖喂养加游泳运动;联合组8只:在运动组基础上加用二甲双胍300 mg/kg.d。6周后,测各组大鼠空腹胰岛素(FINS)、空腹血糖(FPG),计算胰岛素抵抗指数(IRI);酶联免疫吸附法测定血清瘦素,免疫组化法检测肝脏、骨骼肌和脂肪组织中瘦素受体蛋白表达,RT-PCR法检测肝脏、骨骼肌和脂肪中瘦素受体mRNA表达。结果与模型组比较,二甲双胍组、运动组和联合组FPG、FINS水平降低,IRI升高(均P<0.05)。与对照组比较,模型组血清瘦素水平明显升高(P<0.01),肝脏、骨骼肌、脂肪组织中瘦素受体蛋白和mRNA表达均明显下调(均P<0.01);与模型组比较,二甲双胍组、运动组和联合组血清瘦素水平降低(P<0.05),瘦素受体蛋白和mRNA表达均上调(P<0.05)。与二甲双胍组比较,运动组和联合组瘦素受体蛋白及mRNA表达上调(均P<0.05)。结论胰岛素抵抗大鼠存在血清瘦素水平升高,组织瘦素受体表达下调。运动可以改善胰岛素抵抗,可能与降低血清瘦素水平,上调组织瘦素受体蛋白及mRNA表达有关。Objective To investigate the serum lepnn, leptin receptor of tissue in rats with insulin resistance （IR）, and the effect of exer- cise on them. Methods 9 male Sprague-Dawley rats were fed with normal diet as control group. 34 rats were randomly assigned into 4 groups： model group （n=9）, fed with high-fat/high-sucrose diet without any other intervention; metformin group （n=8）, fed with high-fat/ high-sucrose diet and metformin 300 mg/kg, d; exercise group （n=9）, fed with high-fat/high-sucrose diet and trained with swimming; inte- grated group （n=8）, fed with high-fat/high-sucrose diet and metformin 300 mg/kg, d, and trained with swimming. After 6 weeks of interven- tion, the serum leptin was measured with the enzyme-linked immunoabsorbent assay; the expression of leptin receptor protein in liver, skele- tal muscle and adipose were measured with immunohistochemistry, and the expression of leptin receptor mRNA in the same tissues was measured with reverse transcriptase-polymerase chain reaction. Results Compared with the model group, the levels of fasting blood glucose and fasting insulin decreased in the exercise, metformin and integrated group （P〈0.05）, while the 1R index increased （P〈0.05）. Compared with the control group, the serum leptin increased in the model group （P〈0.01）, while the expression of leptin receptor protein and mRNA in liver, skeletal muscle and adipose decreased （P〈0.01）. Compared with the model group, the serum leptin decreased in the exercise, metfor- min and integrated groups （P〈0.05）, while the expression of leptin receptor protein and mRNA increased （P〈0.05）. Compared with the met- formin group, the expression of leptin receptor protein and mRNA increased in the exercise and integrated groups （P〈0.05）. Conclusion High level of serum leptin and low expression of leptin receptor can be found in rats with insulin resistance. Exercise can improve the insu- lin resistance, which may associate with the decrease of the serum lep

关 键 词：胰岛素抵抗 运动 瘦素 瘦素受体 大鼠 

分 类 号：R589.2[医药卫生—内分泌]