基于铽-沙拉沙星配合物的荧光增强测定人血清白蛋白  

Determination of human serum albumin based on fluorescence enhancement of Tb3+-sarafloxacin complexes

作　　者：张敏[1] 于永丽[1] 王丽美[1] 徐淑坤[1] 王乃芝[1] 

机构地区：[1]东北大学理学院,沈阳110819

出　　处：《分析试验室》2013年第2期22-25,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20875011)资助

摘　　要：Tb3+和沙拉沙星(SRFX)反应生成二元配合物,发射Tb3+的特征荧光。人血清白蛋白(HSA)能够增强Tb3+-SRFX配合物的荧光强度,据此,建立了荧光法测定HSA的新方法。在最佳测定条件下,当HSA的浓度在0.50～90.0 mg/L时,Tb3+-SRFX-HSA体系荧光强度的增强和HSA的浓度有良好的线性关系,方法的检出限为0.13 mg/L。用该方法测定了人血清中HSA的含量,回收率在99.2%～99.6%之间。同时对荧光强度增强的机理进行了探讨。Saraflaxcin can combine with Tb^3＋ to form complexes, which emit strong characteristic fluorescence of Tb^3＋. The fluorescence intensities of Tb^3＋ -SRFX complexes could be enhanced by human serum albumin （HSA）. Based on this phenomenon, a simple and sensitive fluorescence method for the determination of HSA was developed. Under the optimum conditions, the fluorescence enhancement of Tb^3＋ -SRFX complexes was proportional to the concentration of HSA in the range of 0.50 -90.0 rag/L, and the detection limit was 0. 13 mg/L. The method was applied to the determination of the content of HSA from human serum samples. The recoveries of 99. 2% - 99.6% showed that the method was accurate and reliable. Additionally, the mechanism of fluorescence enhancement was discussed.

关 键 词：铽 沙拉沙星 荧光 人血清白蛋白 稀土 

分 类 号：O657.3[理学—分析化学]