不同苹果砧木组培苗抗旱性的比较研究  

Drought resistance of different apple rootstocks in vitro

作　　者：张静[1] 赵亮明[1] 邹志荣[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《果树学报》2013年第1期88-93,共6页Journal of Fruit Science

基　　金：国家"十二五"科技支撑项目(2011BAD12B03);国家现代苹果产业技术专项:(2008ZDGC-0)

摘　　要：【目的】为了提供苹果耐旱育种及栽培材料,【方法】以5种苹果砧木组培苗为试材,PEG-6000作为胁迫剂,模拟室外干旱进行处理,运用模糊隶属函数抗旱性综合分析,比较了5个苹果砧木组培苗的耐旱性。【结果】结果表明,在PEG-6000胁迫条件下,不同苹果砧木的叶绿素含量总体均呈下降趋势,叶片的相对电导率和MDA含量随PEG-6000胁迫质量分数的升高逐渐升高。在整个胁迫过程中,各苹果砧木组培苗叶片内蛋白质含量随胁迫质量分数的升高而增大。SOD活性随PEG-6000胁迫质量分数的升高呈先升高后下降趋势,且在20%PEG-6000胁迫条件下SOD活性达到了最大。POD活性随胁迫质量分数的升高呈现出逐渐升高的趋势。【结论】5种苹果砧木的抗旱性顺序依次为:新疆野苹果>平邑甜茶>八棱海棠>‘M26’>‘M9’。【Objective】 The study was to provide drought tolerant apple lines for breeding and cultivation practice.【Method】 Tissue cultured shoots of five apple rootstocks were treated with PEG-6000,simulating field drought stress conditions.Drought tolerance of the shoots was evaluated by meta-analysis using fuzzy membership function.【Result】 The results showed that the chlorophyll content displayed a decreasing tendency under PEG-6000 stress,whereas the relative electric conductivity and malondialdehyde（MDA） content in leaf increased gradually with the increase of PEG-6000 concentration.In the stress treatment,the protein content in leaf of all rootstocks increased with the PEG-6000 concentration.Superoxide dismutases（SOD） activity first increased and then declined with increasing PEG-6000 concentration,with a peak value at 20% PEG-6000.However,the POD activity went up gradually in all the rootstocks with the increase of drought stress strength.【Conclusion】 The drought tolerances of the five rootstocks ranked as follows： Malus sieversii〉M.hupehensis〉M.micromalus＇〉M26＇＇〉M29＇.

关 键 词：苹果砧木组培苗 PEG-6000胁迫 抗旱性评价 

分 类 号：S661.1[农业科学—果树学]