不同菌株绿僵菌对粒肩天牛幼虫的致病力及林间防治效果  

Control efficiency and pathogenicity in different strains of Metarhizium anisopliae on Apriona germari Hope larvae

作　　者：陈德兰 

机构地区：[1]武夷山市林业局,福建武夷山354300

出　　处：《南京林业大学学报（自然科学版）》2013年第1期78-82,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：福建省科技厅重点科技资助项目(2004N013);南平市科技局科技资助项目(N2005Z12)

摘　　要：粒肩天牛是福建省杨树林的一种主要害虫,此次研究应用7株金龟子绿僵菌菌株对该天牛幼虫进行生物测定,旨在筛选出感染该虫的高致病力菌株,为生物防治提供新的资源。结果表明:感染Ma1291-2和Ma1775两个绿僵菌菌株14 d后,天牛幼虫的校正死亡率分别为89.47%和88.92%;Ma1291-2和Ma1775两个绿僵菌株对天牛幼虫的致死中时间(LT50)分别为5.62和5.82 d,孢子致死中浓度(LC50)分别为1.70×104个/mL和6.16×104个/mL。说明绿僵菌Ma1291-2和Ma1775两个菌株对该天牛幼虫的致病力强,不仅致死率高,且致死速度快,僵虫率高,应以浓度为1.5×108个/mL的孢子悬浮液进行林间防治,效果良好。Apriona germari Hope is a main insect pest of poplar in Fujian province.In order to screen high virulence strains from Metarhizium anisopliae against A.germari larvae,bioassays from seven strains of M.anisopliae were carried out through dipping in spore suspension.The results showed that the cumulative corrected mortality of A.germari larvae inoculated with Ma1291-2 and Ma1775 strains reached to 89.47% and 88.92% after 14 d treatment.While LT50 value of the two strains were 5.62 d and 5.82 d,and LC50 value were 1.70×104spore/mL and 6.16×104spore/mL,respectively.It could be concluded that Ma1291-2 and Ma1775 had higher virulence than the other strains,with rapid growth and high sporulation.A separate field bioassay suggested that Ma1291-2 and Ma1775 could be used to control A.germari larvae,with 1.5×108 spore/mL.

关 键 词：金龟子绿僵菌 粒肩天牛 致病力 防治 杨树 

分 类 号：Q965.8[生物学—昆虫学] S763.3[农业科学—森林保护学]