盐胁迫对两种忍冬属植物活性氧平衡的影响  

Reactive oxygen species homeostasis of two Lonicera species under salt stress

作　　者：程淑娟[1] 唐东芹[1,2] 刘群录[1,2] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海城市植物资源开发应用工程技术研究中心,上海200231

出　　处：《南京林业大学学报（自然科学版）》2013年第1期137-141,共5页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：“十一五”国家科技支撑计划(2008BAJ10B04);上海市科委项目(09dz1205003)

摘　　要：以红白忍冬和郁香忍冬盆栽苗为试验材料,研究不同浓度的NaCl胁迫对两者生理指标的影响,并比较它们的耐盐性差异。结果表明,在盐胁迫条件下,两种植物叶片的电导率、MDA含量均不断上升。胁迫期间,红白忍冬的H2O2含量相对于对照没有明显变化,O.2-产生速率在处理前期明显上升,上升出现的时间迟于郁香忍冬;而郁香忍冬的H2O2含量一直维持较高水平,O.2-产生速率在处理前期也有所增加。红白忍冬的SOD、APX和CAT活性总体表现出先上升后下降的趋势,而POD活性则一直上升。郁香忍冬在胁迫期间,其SOD活性有小幅增加;APX和CAT的活性均呈现先上升后下降的趋势;POD活性前期没有明显增加,在处理终期时也仅在100mmol/L胁迫下才显著增加。表明两种植物在遭受盐胁迫时,均能启动抗氧化酶系统来清除活性氧,降低氧化胁迫的伤害。同时,相对于郁香忍冬,红白忍冬通过快速上调抗氧化酶活性,降低盐胁迫对膜脂过氧化作用,保持膜的完整性,从而更好地抵御氧化损害更耐受盐胁迫。Lonicera japonica var.chinensis and L.fragrantissima potted plants were used to study their physiological response and salt tolerance under NaCl stress.The results showed that the electrical conductivity and MDA content increased with the increasing of salt concentration in both species.There was no obvious rise of H2O2 content in L.japonica var.chinensis,while a higher level of H2O2 content in L.fragrantissima was observed than control.There was a significant generating rate of superoxide radical（O-· 2） at early stage of salt stress in L.japonica var.chinensis,but the time was later than superoxide radical in L.fragrantissima.For L.japonica var.chinensis,the activities of SOD,APX,and CAT increased and then decreased,while the POD activity showed a constant increase during stress process.For L.fragrantissima,the SOD activity increased slightly during NaCl stress,however,the activities of APX and CAT increased first and then decreased.No obvious POD activity increment was detected,only a significant increment was observed under at the end of 100 mmol/L NaCl stress.These results suggested that the two Lonicera species could employantioxidant enzymes to eliminate ROS under salt stress.Meanwhile,the salt tolerance of L.japonica var.chinensis was better than that of L.fragrantissima by rapidly increasing the activities of antioxidant enzymes to decrease the lipid peroxidation level and reduce ROS damage.

关 键 词：红白忍冬 郁香忍冬 盐胁迫 活性氧 抗氧化酶 

分 类 号：S718[农业科学—林学]