阿托伐他汀对野百合碱诱导的大鼠肺动脉高压及肺组织炎症的影响  

The effects of atorvastatin in ameliorating monocrotaline(MCT)-induced pulmonary hypertension in rats through reduction of inflammation

作　　者：邢西迁[1] 吴绪伟[1] 吴尚洁[2] 李艳丽[1] 张红艳[1] 李志东[1] 魏星[1] 肖谊[1] 

机构地区：[1]昆明医学院附属延安医院呼吸一科,昆明650051 [2]中南大学湘雅二医院呼吸科

出　　处：《现代预防医学》2013年第3期517-520,共4页Modern Preventive Medicine

基　　金：国家自然科学基金资助项目(81100037);云南省应用基础研究计划项目(2011FB154);云南省教育厅科学研究基金项目(2010C091)

摘　　要：目的观察阿托伐他汀对野百合碱(monocrotaline,MCT)诱导的大鼠肺动脉压及肺部炎症的影响。方法将24只SD大鼠随机分为正常对照组(对照组),MCT诱导的肺动脉高压组(PH模型组),阿托伐他汀干预组(治疗组),每组8只。PH模型组和治疗组分别一次性腹部皮下注射MCT(60mg/kg),治疗组自注射MCT之日起以阿托伐他汀(10mg/kg)灌胃,每日1次。3周后达实验终点,测定大鼠平均肺动脉压、计算右心室肥大指数。肺组织切片进行HE染色,取肺组织观察肺小动脉周围炎症细胞浸润程度,并计算肺中、小动脉管壁厚度占血管外径的百分比(WT%)和肺动脉管壁面积/管总面积的百分比(WA%),以此反映肺血管重建情况。结果治疗组大鼠的平均肺动脉压、右心室肥大指数、肺中、小动脉WT%和WA%均较PH模型组明显减小,血管周围炎明显减轻(P均﹤0.01)。大鼠肺组织炎性细胞计数与平均肺动脉压、肺中、小动脉WT%和WA%均呈明显正相关(P均﹤0.01)。结论阿托伐他汀具有抑制MCT诱导的肺部炎症、肺血管重建和肺动脉高压形成的作用。OBJECTIVE To investigate the effects of atorvastatin on monocrotaline （MCT） -induced pulmonary hypertension in rats and the inflammation in lung. METHODS Twenty-four male SD rats were randomly divided into three groups including control group, MCT group and atorvastatin group （AS group）. There were eight rats in each group. The rats in MCT and AS group were given a single subcutaneously injection of MCT （60mg/kg）. Atorvastatin （10mg/kg/d） were given orally for 21 days to the rats in AS groups since the day when rats were injected MCT. At 22 days. Mean pulmonary arterial pressure （mPAP） and right ventricular hypertrophy index （RVHI） were measured. Perivascular inflammation was observed. The index of wall thickness of pulmonary arteriole was measured by a computerized image analyzer. RESULTS The mPAP and RVHI increased significantly in MCT group than that in control group （P 〈 0.01）. This increase in mPAP and RVHI was partially prevented by atorvastatin （P 〈 0.01 ）. Atorvastatin treatment was associated with a significant reduction of MCT-induced number of inflamma- tory cells. This increase in WT% and WA% was partially prevented by atorvastatin （P 〈 0.01 ）. The percentage of inflammatory cells was positively correlated with mPAP and WT% and WA% of pulmonary medial arteries and arteriole （P 〈 0.01, respec- tively）. CONCLUSION Atorvastatin could prevent MCT-induced inflammatory response, pulmonary vascular remodeling and the development of pulmonary hypertension.

关 键 词：高血压 肺性 他汀类药物 炎症 

分 类 号：R972.4[医药卫生—药品]