耐甲氧西林金黄色葡萄球菌中mecA、femA检测及其抗生素敏感性研究  

The detection on mecA and femA genes of methicillin resistant staphylococcus aureus(MRSA)strains and study of antimicrobial sensitivity

作　　者：许顺姬[1] 许东哲[1] 

机构地区：[1]吉林延边大学医院,吉林延吉133000

出　　处：《现代预防医学》2013年第3期531-532,535,共3页Modern Preventive Medicine

摘　　要：目的探讨mecA、femA基因PCR聚合扩散快速检测耐甲氧西林金黄色葡萄球菌(MRSA)的敏感性及特异性。方法选择耐甲氧西林金黄色葡萄球菌株84株用PCR聚合扩散法检测mecA、femA基因,并用纸片扩散法检测MRSA菌株的药物敏感性。结果 84株耐甲氧西林金黄色葡萄球菌中,mecA基因阳性率为100%femA基因阳性率为97.6%。未发现万古霉素耐药的菌株。结论 PCRmecA、femA基因聚合扩散法即可用于MRSA快速诊断,又有特异性强的有点。OBJECTIVE To discuss PCR speed assay in detection of meeA, femA genes, sensitivity and specificity of methieillin resistant staphylococcus aureus （MRSA）. METHODS A total of 84 strains of methicillin resistant staphylococcus aureus were selected to test meeA, femA genes with PCR assay and to detect antimierobial sensitivity of MRSA strains with disk diffusion. RESULTS Of the 84 strain of MRSA, positive rate of meeA gene was 84/84 （ 100% ） and femA gene was 82/84 （97.6%）. There were no resistance strains to vancomycin. CONCLUSION The detection of MecA and femA genes by PCR assay is not only speedy for MRSA, but also with high sensitivity and specificity.

关 键 词：耐甲氧西林金黄色葡萄球 MECA基因 FEMA基因 抗生素敏感性 

分 类 号：R378.11[医药卫生—病原生物学]