新诊断2型糖尿病患者外周血单个核细胞中JNK活性变化及其影响因素  

JNK activity in peripheral blood mononuclear cells of newly diagnosed type 2 diabetic patients and its influencing factors

作　　者：刘德英 路文盛[2] 

机构地区：[1]凌云县人民医院内科,广西533100 [2]广西壮族自治区人民医院内分泌代谢科,南宁530021

出　　处：《中国临床新医学》2013年第1期5-7,共3页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：国家自然科学基金资助项目(编号:30860113);广西卫生厅重点科研课题(编号:桂卫重200736)

摘　　要：目的了解新诊断2型糖尿病外周血单个核细胞中氨基末端激酶(JNK)(Thr183/Tyr185)活性变化及其影响因素。方法招募正常糖耐量健康志愿者60名(NGT组),新诊断2型糖尿病患者60例(NT2DM组)。应用westernblot法检测外周血单个核细胞中JNK(Thr183/Tyr185)磷酸化水平。结果 NT2DM组JNK(Thr183/Tyr185)磷酸化水平显著高于NGT组,差异有统计学意义(P<0.01)。相关性分析显示,JNK(Thr183/Tyr185)磷酸化水平与体重指数(BMI)、空腹血糖(FBG)、低密度脂蛋白胆固醇(LDL-C)呈正相关(P<0.05),在剔除年龄、性别等相关影响因素之后,空腹血糖仍与JNK(Thr183/Tyr185)磷酸化水平独立相关。结论 JNK通路过度激活可能增加了患糖尿病的风险。Objective To explore the change of JNK （Thr^183/Tyr^185） activity in the peripheral blood mono- nuclear cells in the newly diagnosed type 2 diabetes patients. Methods A total of 60 healthy volunteers with normal glucose tolerance （NGT group）, 60 newly diagnosed type 2 diabetes patients （NT2DM group） were recruited in our study. JNK （Thr^183/Tyr^185 ） phosphorylation levels were deleeted in the peripheral blood mononuclear cells by western blot method. Results JNK （ Thr^183/Tyr^185 ） phosphorylation levels in NT2DM group were significantly higher than that in NGT group （P 〈 0. 01 ）. The correlation analysis showed that JNK （Thr^183/Tyr^185） phosphorylation levels were positively associated with body mass index, fasting blood glucose and low-density cholesterol （ P 〈 0. 05 ）. Under the situation of excluding the related factors such age, gender and so on, JNK （ Thr^183/Tyr^185 ） phosphorylation levels were still independently associated with fasting blood glucose （ P 〈 0. 05）. Conclusion JNK pathway over-activation may increase the risk of diabetes.

关 键 词：JNK 2型糖尿病 单个核细胞 胰岛素抵抗 相关性分析 

分 类 号：R587.1[医药卫生—内分泌]