黄芪甲苷对大鼠海马源神经干细胞体外增殖的影响  

Influence of astragaloside on proliferation of neural stem cells derived from hippocampus in vitro

作　　者：江清林[1] 张天英[2] 李竹[3] 辛华[4] 齐志国[5] 盛宝英[5] 朱晓峰[1] 

机构地区：[1]佳木斯大学神经科学研究所,154003 [2]佳木斯大学附属第一医院药剂科 [3]佳木斯大学附属第一医院供应室 [4]佳木斯大学附属第一医院检验科 [5]佳木斯大学附属第一医院神经科

出　　处：《中国医药》2013年第2期179-181,共3页China Medicine

基　　金：黑龙江省教育厅科学技术研究项目（12521543）

摘　　要：目的观察黄芪甲苷对神经干细胞（NSC）体外增殖的影响，为神经干细胞应用于临床提供理论依据。方法利用无血清培养技术，从新生大鼠海马区分离培养NSC，并进行体外扩增、传代。取传至第3代的NSC，观察不同浓度黄芪甲苷（40、80、120mg／L）对其增殖的影响。对神经球形成数进行计数并进行噻唑蓝（MTT）比色分析。结果40mg／L和80mg／L黄芪甲苷实验组神经球形成数和细胞吸光度值均明显高于对照组（神经球形成数：8．1±1．4，8．8±1．3比7．2±1．2；细胞吸光度值：0．87±0．02、0．89±0．03比0．694-0．03，P〈0．05或P〈0．01），而120mg／L黄芪甲苷组实验组神经球形成数和细胞吸光度值（分别为3．1±1．2、0．30±0．02）低于对照组（P〈0．01）。结论在一定浓度范围内，黄芪甲苷可促进体外培养NSC的增殖。Objective To observe the influence of astragaloside on proliferation of neural stem cells （NSCs） in vitro to provide a theoretical basis for clinical application of NSCs. Methods Through serum-free culture technology, NSCs were isolated from the hippocampus of neonatal rats and cultured, and were amplified, passaged. Check spread to the third generation of neural stem cells, the effects of different concentrations of astragaloside （40, 80, 120 mg/L） for their proliferation were observed. The number of neurospheres formed were counted and MTF colorimetric analysis were conducted. Results The number of neurospheres formed and the optical density （A） in 40 mg/L and 80 mg/L astragaloside group were significantly higher than those in control group （8.1 ±1.4, 8.8 ±1.3 vs 7.2 ±1.2, 0.87 ±0.02, 0.89 ±0.03 vs 0.69 ±0.03, P 〈0.05 or P 〈0.01）, while those in 120 mg/L astragaloside group （ 3.1 ± 1.2, 0.30 ± 0.02, respectively） were significantly lower than those in control group. Conclusion In a certain range of concentration, astragaloside can promote proliferation of neural stem cells in vitro.

关 键 词：黄芪甲苷 神经干细胞 增殖 

分 类 号：R285.5[医药卫生—中药学]