PNIPAM温敏微凝胶在生物医学领域中的应用研究  

Applications of Thermosensitive PNIPAM Microgels in Biomedical Field

作　　者：张拥军[1] 关英[1] 罗巧芳 刘鹏霄 甘添天 王东东 邢淑滢 廖望 张娅彭 程丹 

机构地区：[1]功能高分子材料教育部重点实验室,南开大学化学学院高分子化学研究所,天津300071

出　　处：《高分子通报》2013年第1期26-39,共14页Polymer Bulletin

基　　金：国家自然科学基金(Nos.20744001;20774049;20974050;21174070);科技部国际科技合作项目(No.2007DFA50760);天津市科技支撑计划国际科技合作项目(合同号:07ZCGHHZ01200);教育部"新世纪优秀人才支持计划"(No.NCET-11-0264)资助

摘　　要：水凝胶因其良好的生物相容性及环境刺激响应性而在生物医学领域有着广泛的用途,但仍存在机械强度差、响应速度慢、不能生物降解等缺点。针对这些问题,特别是宏观水凝胶响应慢的问题,我们近年来以具有温度敏感性的聚N-异丙基丙烯酰胺(PNIPAM)微凝胶为基础,设计制备了一系列生物材料,分别应用于药物控释、生物传感以及组织工程等生物医学领域。我们设计制备了具有良好葡萄糖敏感性的PNIPAM微凝胶,实现了可自我调控的胰岛素可控释放。以PNIPAM微凝胶为基础,提出了新的聚合胶态晶体阵列光学传感方法,设计制备了多种可快速响应的新型生物光学传感器。实现了PNIPAM微凝胶的实时凝胶化,并将其发展成为一种新型的可注射细胞支架材料。进一步利用该体系的可逆性,提出了制备在药物筛选、肿瘤研究以及组织工程等领域有重要用途的多细胞球的新方法。Hydrogels have found wide applications in biomedical field because of their good biocompatibility and stimuli- responsivity, however, some drawbacks, such as weak mechanical strength, slow response rate and lack of biodegradability, still need to be resolved. In recent years, we designed a series of biomaterials, mainly based on the thermosensitive poly （N- isopropylacrylamide） （PNIPAM） microgels, to be used as drug carriers, biosensors and cell scaffolds. The major problem we aimed to resolve is the slow response of the hydrogel biomaterials. We have designed and prepared PNIPAM rfiicrogels with good glucose-sensitivity, which were used for self-regulated insulin release. We proposed a new polymerized crystalline colloidal array optical sensing method and developed several optical biosensors which can respond quickly. We also achieved the in situ gelation of concentrated PNIPAM microgel dispersions, which were further developed as a new injectable 3D cell scaffold. Also based on the reversibility of this system, we proposed a new method for the fabrication of multicellular spheroids, which have important applications in drug screening, cancer study and tissue engineering.

关 键 词：水凝胶 微凝胶 药物控释 生物传感 细胞支架 

分 类 号：TQ460.1[化学工程—制药化工]