纳米结构蛋白质分子印迹聚合物的研究进展  

An Overview of Nanostructured Protein Imprinted Polymers:Synthesis and Applications

作　　者：董明杰[1] 李义雅[1] 柴志华[1] 袁冬英[1] 傅国旗[1] 

机构地区：[1]南开大学高分子化学研究所,功能高分子材料教育部重点实验室,天津300071

出　　处：《高分子通报》2013年第1期127-136,共10页Polymer Bulletin

基　　金：国家自然科学基金(21074061;20574038);天津市自然科学基金(No.09JCYBJC02900)

摘　　要：在生物技术和生命科学等领域常依赖抗体的特异识别作用来实现目标蛋白质的分离、分析和检测。以蛋白质为模板的分子印迹聚合物有望取代这些昂贵的抗体,但交联聚合物网络中大分子的传质限制是蛋白质分子印迹研究中面临的一个重要问题。纳米结构的蛋白质印迹材料具有较高的表面积与体积比及较浅的印迹位点,很大程度上可克服这一问题。本文详细介绍各种纳米蛋白质印迹聚合物的制备方法,分析各自的优势和不足,并简要介绍其应用。In the areas such as biotechnologies and life science, separation, analysis and detection of a target protein is achieved commonly relying on antibody-based specific interactions. Molecularly imprinted polymers against proteins are regarded as the most potential substitutes for the costly antibodies. However, successful imprinting of a protein is faced with some problems, one of which is the limited macrornolecule diffusion throughout the cross-linked protein-imprinted polymer networks. Nanostructured protein-imprinted polymers can overcome this problem to a great extent, with relatively high ratio of surface area to volume and easily accessed imprinting sites. Herein, we review various approaches reported previously for constructing the nanosized protein-imprinted materials, particularly addressing the advantages and shortcomings associated with each method. The potential applications of these protein-imprinted materials are also described.

关 键 词：分子印迹 蛋白质印迹 蛋白质识别 纳米结构 

分 类 号：Q51[生物学—生物化学] TB383.1[一般工业技术—材料科学与工程]