阿托伐他汀对缺血再灌注损伤下内皮祖细胞的保护作用  

Protective effects of atorvastatin on endothelial progenitor cells against ischemia-reperfusion injury

作　　者：梁伟钧[1] 王苗[1] 张日霖[1] 李上海[1] 

机构地区：[1]广东医学院附属医院心内科,湛江524000

出　　处：《国际心血管病杂志》2013年第1期49-52,共4页International Journal of Cardiovascular Disease

基　　金：广东省科技计划项目(2009B030801337)

摘　　要：目的:探讨阿托伐他汀对缺血再灌注损伤下内皮祖细胞(EPCs)的保护作用及其机制。方法:分离SD大鼠骨髓中单个核细胞,采用差速贴壁法纯化,间接免疫荧光法鉴定EPCs。将EPCs随机分成对照组(C组)、缺血再灌注组(IR组)、阿托伐他汀组(A组)、缺血再灌注/阿托伐他汀组(IR/A组)及阿托伐他汀/缺血再灌注组(A/IR组)。采用MTT法测定EPCs增殖情况,并检测细胞培养液中乳酸脱氢酶(LDH)释放量及一氧化氮(NO)及总一氧化氮合酶(TNOS)分泌量。结果:与C组比较,IR组EPCs增殖减慢,NO和TNOS分泌量减少,LDH释放量明显增加。经阿托伐他汀预处理后,与IR组相比,EPCs增殖快,NO及TNOS分泌量升高,LDH释放量减少。结论:阿托伐他汀通过促进EPS增殖和NO分泌,对缺血再灌注损伤状态下的EPCs有保护作用。Objective:To investigate the protective effects of atorvastatin on endothelial progenitor cells(EPCs) undergoing ischemia-reperfusion injury and the possible mechanisms.Methods:Mononuclear cells from the bone marrow of SD rats were isolated by density gradient centrifugation,purified by selective plating technique and identified by indirect immunofluorescence assay.EPCs were divided randomly into five groups: control group(Group C),ischemia-reperfusion group(Group IR),Atorvastatin group(Group A),ischemia-reperfusion/atorvastatin group(Group IR/A) and atorvastatin/ischemia-reperfusion group(Group A/IR).The proliferation of EPCs was detected by MTT assay.The secretions of LDH,NO and TNOS in the cell-culture medium were measured.Results:The number of EPCs and the secretions of NO and TNOS decreased,while the secretion of LDH increased significantly in the ischemia-reperfusion model compared with group C(P<0.05).However,the number of EPCs and the secretions of NO and TNOS increased,the secretion of LDH decreased after the pretreatment of atorvastatin compared with group IR(P<0.05).Conclusion:Atorvastatin promotes proliferation and secretion of NO in the endothelial progenitor cells,which may be related to its effect on the secretion of TNOS.

关 键 词：阿托伐他汀 缺血再灌注损伤 内皮祖细胞 

分 类 号：R96[医药卫生—药理学]