自动净化及新型酶联免疫法测定废气中的二噁英  

An Analytical Method Based on Automatic Sample Preparation and New Enzyme-linked Immunosorbent Assay and Its Applications in Determination of Dioxin in Exhaust Samples

作　　者：周志广[1] 许鹏军[1] 任玥[1] 李楠[1] 齐丽[1] 郑森[1] 赵虎[1] 范爽[1] 张烃[1] 刘爱民[1] 黄业茹[1] 高木阳子 

机构地区：[1]国家环境分析测试中心国家环境保护二噁英污染控制重点实验室,北京100029 [2]京都电子工业株式会社

出　　处：《分析测试学报》2013年第1期127-132,共6页Journal of Instrumental Analysis

基　　金：国家环保公益性科研专项"城市环境二噁英监测技术规范与快速监测技术研究"(200909095);国家重点基础研究计划(973)项目(2009CB421602)

摘　　要：研究了一种适用于二噁英生物检测法的样品前处理仪器(SPD-600),考察了不同条件下的净化效果和内标回收率,建立了一种新型酶联免疫系统(DXS-600)测定二噁英的方法。研究表明,SPD-600在60℃加热的条件下净化效果最好,杂质基本被去除,内标回收率与人工制备的多层硅胶柱净化的回收率基本相当;DXS-600采用平衡除外的方法,避免了常规酶联免疫方法中的竞争步骤,消除了实验过程中的人为不确定因素,从而提高了灵敏度和稳定性;采用SPD-600净化、DXS-600测定的组合分析了21个废弃物焚烧废气样品,并与高分辨气相色谱-高分辨质谱的测定结果进行比较。结果表明,两种方法测定结果之间具有很高的相关性(r2=0.9858)。SPD-600净化结合DXS-600测定方法可作为二噁英的快速筛查方法。The application of a pretreatment instrument,SPD-600,in dioxin-like substances determination by a new enzyme-linked immunosorbent assay method was introduced in this paper,Its clean-up efficiencies and recoveries under different conditions were evaluated.The results indicated that SPD-600 achieved the optimum performance under a heated condition of 60 ℃ when almost all impurities were removed.And the recoveries of the labeled internal standards are comparable with that of the multilayer silica column treatment.In addition,an ELISA-based kinetic exclusion assay method using an automatic analysis instrument（DXS-600） was developed for dioxin analysis,which had avoided the competitive step.The results showed that DXS-600 eliminated the artificial effects and was more sensitive and stable than the traditional ELISA.Finally,21 flue gas samples were determined using SPD-600 combined with DXS-600 systems,and the results obtained were compared with those of the HRGC-HRMS analysis.A good correlation with r2 of 0.985 8 was found between two methods.Therefore,the method of SPD-600 clean-up combined with DXS-600 analysis can be used as a quick screening method for the determination of dioxin.

关 键 词：SPD-600 DXS-600 高分辨气相色谱-高分辨质谱(HRGC-HRMS) 二噁英 

分 类 号：O657.7[理学—分析化学] TQ612.1[理学—化学]