丹酚酸B对血小板聚集及释放sP-sel和sCD40L的影响  

Effect of Salvianolic Acid B on the Platelet Aggregation and sP-sel and sCD40L Release

作　　者：李伟[1] 张军平[2] 徐士欣[2] 仲爱芹[1] 华声瑜[1] 

机构地区：[1]天津中医药大学,天津300193 [2]天津中医药大学第一附属医院,天津300073

出　　处：《中华中医药学刊》2013年第1期48-50,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30801499);高等学校博士学科点专项科研基金资助项目(20111210120010)

摘　　要：目的:探讨丹酚酸B(SaIB)对二磷酸腺苷(ADP)和凝血酶(Th)诱导的血小板聚集率以及释放的可溶性P选择素(sP-sel)和可溶性CD40L(sCD40L)的影响。方法:采集健康志愿者静脉血,制备富血小板血浆(PRP),以终浓度10μmol/L ADP和0.4 U/mL Th为诱导剂,测定血小板聚集率,分别以300μg/mL和600μg/mLSalB预孵育血小板,测定聚集抑制率。制备血小板悬液,分为静息组、激活组(以终浓度50μmol/L ADP和0.4U/mL Th为诱导剂激活)、SalB组(以终浓度300μg/mL和600μg/mL SalB预孵育,再以诱导剂激活)。采用ELISA方法检测各组上清液中sP-sel和sCD40L含量,比较sP-sel和sCD40L的相关性。结果:SalB可显著减低ADP和Th诱导的血小板聚集率,600μg/mL组聚集抑制率高于300μg/mL组(P<0.01)。与静息组比较,激活组sP-sel和sCD40L释放显著增加(P<0.01)。与激活组比较,SalB可使Th诱导的sP-sel和sCD40L释放及ADP诱导的sP-sel释放显著降低(P<0.01);但对ADP诱导的sCD40L释放没有影响,与激活组比较差异无统计学意义(P>0.05)。ADP和Th诱导时,sP-sel与sCD40L水平呈正相关(P<0.05,P<0.01),相关系数r_P>0.5。结论:SalB可降低体外血小板聚集率及活化产物sP-sel和sCD40L的释放,具有阻抑血小板活化、聚集与释放的作用。Objective：To explore the effect of Salvianolic acid B（SalB） on platelet aggregation,soluble P-selectin （sP-sel）and soluble CD40L（sCD40L） release by activated platelets which were respectively induced by adenosine diphosphate（ADP）and thrombin（Th）.Methods：Platelet Rich Plasma（PRP） collected from healthy volunteers,was induced respectively by ADP（10μmol/L）and Th（0.4 U/mL）for platelet aggregation rate.Inhibition rate was assayed under the pre-incubation of SalB（300μg/mL,600μg/mL）.PLT suspension liquid was divided into four groups：resting group,activating group[induced by ADP（50μmol/L） and Th（0.4 U/mL）]and SalB group（terminal concentration of 300μg/mL and 600μg/mL,pre-incubate and activated by inducers）.sP-sel and sCD40L contents in each supernatant were assayed by ELISA to compare the correlation of sP-sel and sCD40L.Results：SalB can significantly reduce the rate of platelet aggregation by ADP and Th induction.SalB group（600μg/mL） inhibition rate was higher than that of 300μg/mL group（P0.01）.Compared with resting group,release of sP-sel and sCD40L in activating group significantly increased（P0.01）.Compared with activating group,SalB group（sP-sel and sCD40L induced by Th） and SalB group （sP-sel induced by ADP） were significantly reduced（P0.01）;but the difference had no statistical significance between SalB group（sCD40L induced by ADP） and activating group（P0.05）.The contents of sP-sel and sCD40L induced by ADP were positively correlated with those by Th（P0.05,P0.01）,r_P0.5.Conclusions：SalB can reduce platelet aggregation rate in vitro and sP-sel and sCD40L which are released by activated platelets.SalB can inhibit platelet activation,aggregation and release.

关 键 词：丹酚酸B 活化血小板 可溶性P选择素 可溶性CD40L 

分 类 号：R285.5[医药卫生—中药学]