山核桃叶总黄酮制备  

Total Flavonoids Preparation of the Carya cathayensis Sarg leaves

作　　者：朱学鑫[1] 李伟平[1] 俞燕[1] 蒋福升[1] 丁志山[1] 

机构地区：[1]浙江中医药大学,杭州浙江310053

出　　处：《中华中医药学刊》2013年第1期147-149,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省新苗人才计划项目(2008R40G2120013);浙江省卫生厅科研资助项目(2013KYB136);浙江省大学生科技创新计划项目(2010R410002)

摘　　要：目的:对山核桃叶总黄酮的制备工艺进行了系统考察,为后继药效学研究奠定基础。方法:通过正交实验对黄酮提取工艺进行优化,以D101大孔树脂为吸附材料,对其静态吸附容量及黄酮洗脱富集条件进行考察,确定山核桃叶总黄酮制备工艺。结果:山核桃叶总黄酮的最佳提取工艺为:60%乙醇提取2次;提取时间分别为80 min、60 min;溶媒倍数分别为10倍、8倍。D101型树脂对山核桃叶总黄酮的静态吸附容量为(36.78±2.5)mg/g,洗脱次序为20%乙醇洗脱后收集40%乙醇洗脱部分即为山核桃叶总黄酮,其总黄酮得率为(74.93±2.10)%,纯度达(74.07±2.61)%。结论:所确立的山核桃叶总黄酮提取、纯化工艺合理、稳定,黄酮得率及纯度较高,为深入研究山核桃叶总黄酮奠定了良好基础。Objective：To prepare the total flavonoids of Carya cathayensis Sarg leaves（CCL） and settle foundation for its further pharmacology research.Methods：Orthogonal experimental design was applied to optimize the total flavonoids extract technology;static adsorptive capacity and flavonoids enriching condition were investigated on D101 macroporous resin. Results：The final optimized extraction technology was：CCL powder extracted with 10 and 8 times volume of 60%ethanol twice,with reflux time 80 min and 60 min respectively.Total flavonoids of CCL was prepared on D101 resin with static adsorptive capacity（36.78±2.5）mg/g;after eluting with 20%ethanol and collecting the 40%ethanol elute part,the total flavonoids yield was（74.93±2.10）%and with purity of（74.07±2.61）%detected by AlCl_3 colorimetric method. Conclusion：Data above indicated that the method established for preparation of the total flavonoids of CCL was rational and stable,which can pave the way for its further pharmacodynamics research.

关 键 词：山核桃 总黄酮 制备 大孔树脂 

分 类 号：R284.2[医药卫生—中药学]