纳米二氧化硅体内与体外氧化应激及细胞毒性实验研究  

In Vitro and in Vivo Oxidative Stress and Cytotoxicity Induced by Nano-silica

作　　者：史宏博[1] 王尊[1] 于宏升[2] 刘魁[1] 岳霞[1] 郑茹茹[1] 邹宝波[1] 赵进顺[1] 

机构地区：[1]宁波大学医学院,浙江宁波315211 [2]宁波大学医学院附属医院,浙江宁波315020

出　　处：《宁波大学学报（理工版）》2013年第1期127-132,共6页Journal of Ningbo University：Natural Science and Engineering Edition

基　　金：国家自然科学基金(81273111);宁波市自然科学基金(2012A610185);宁波大学新苗计划(2011R405035)

摘　　要：研究了纳米二氧化硅(SiO2)体内及体外氧化应激损伤及细胞毒性作用及机制.体内实验采用ICR小鼠,纳米SiO2经口灌胃染毒3次,检测肺组织中MDA(丙二醛)和SOD(超氧化物歧化酶)含量,观察纳米SiO2对小鼠肺的氧化应激毒性.采用体外培养的小鼠上皮细胞(JB6细胞),观察不同质量浓度纳米SiO2染毒后的细胞形态改变,通过蛋白免疫印迹实验检测纳米SiO2是否通过FAS信号途径引起细胞凋亡.研究结果表明:纳米SiO2经口染毒后对小鼠肺具有氧化应激损伤作用,表现为随染毒剂量的升高,肺组织中MDA产生量增加,呈一定的剂量-反应关系.同时,肺组织内SOD水平总体趋势降低,但是差别未见统计学意义.体外培养细胞实验中,纳米SiO2染毒后可引起细胞出现凋亡并呈空泡样变.蛋白免疫印迹实验中,纳米SiO2可引起FAS信号蛋白表达量明显上升,但是FADD表达水平并不升高.To elucidate the mechanisms of nano-silica induced cytotoxicity and oxidative stress in vivo and in vitro. ICR mice were contaminated using nano-silica three times by oral gavage. The level of MDA （malondialdehyde） and SOD （superoxide dismutase） in the lung tissue were detected as in vivo toxicity endpoints for nano-silica induced oxidative stress. In vitro, mouse epithelial cells （JB6 cells） were cultured, and observed for morphologic changes after treatment with different concentrations of nano-silica. Induction of apoptosis by nano-silica via the FAS signaling pathway was examined by western-blot assay. The result shows that nano-silica induced oxidative stress in mice after oral exposure which is indicated by a significant increase of MDA in lung tissue in a dose-response manner. The SOD levels in lung tissue were decreased. Nano-silica caused oxidative damage in JB6 cells exposed to certain dose ranges. Cell apoptosis were also observed in cells stained with YO-PRO-1 dye. Western blot shows a significant increase in the expression of FAS signaling protein induced by nano-silica. However the level of FADD expression was decreased.

关 键 词：纳米二氧化硅 小 鼠 JB6细胞 氧化应激 细胞凋亡 FAS 

分 类 号：R318[医药卫生—生物医学工程]