蓝萼香茶菜总二萜对小鼠抗凝血作用  

Anticoagulant effect of total diterpenoids in Isodon japonica(Burm.f.) Hara var.glaucocalyx(Maxim.) Hara in mice

作　　者：朱晓红[1] 张冰月[1] 王莹莹[1] 王强[1] 

机构地区：[1]中国人民解放军第二○二医院药剂科,辽宁沈阳110003

出　　处：《中国公共卫生》2013年第1期86-87,共2页Chinese Journal of Public Health

基　　金：辽宁省博士科研启动基金(20091097)

摘　　要：目的探讨蓝萼香茶菜乙醇提取物蓝萼香茶菜总二萜对小鼠抗凝作用影响。方法将小鼠随机分为空白对照组、阳性药物组以及蓝萼香茶菜总二萜组(包括加热回流法和冷浸法)高、中、低剂量组,灌胃给药,连续7 d;通过鼠尾横切法和毛细玻璃管法测定小鼠的凝血时间和出血时间。结果蓝萼香茶菜总二萜(加热回流法、冷浸法)高剂量组小鼠凝血时间分别为(425±13.32)和(559±36.17)s,均长于空白对照组的(201±18.52)s;蓝萼香茶菜总二萜(加热回流法、冷浸法)高剂量组小鼠出血时间分别为(3 308±271.33)和(3 758±184.53)s,均长于空白对照组的(2 094±58.59)s。结论蓝萼香茶菜总二萜剂量依赖性延长小鼠毛细管凝血时间和尾尖出血时间;蓝萼香茶菜总二萜具有抗凝血活性。Objective To explore anticoagulant effect of total diterpenoids extracted with ethanol from Isodon japonica（Burm.f.） Hara var.glaucocalyx（Maxim.） Hara in mice.Methods A total of 90 mice were randomly divided into blank control group,positive drug group,and high,medium,low dose total diterpenoids groups.The different treatments were conducted with gavage administration once a day for 7 days.Tail transection and capillary glass tube method were used to determine clotting time（CT） and bleeding time（BT）.Results The CT was 425.00±13.32 seconds and 559±36.17 seconds for the mice treated with high dose total diterpenoids extracted with heating reflux and cold soak method,respectively,and significantly longer than that of the blank control（201.00±18.52 seconds）.The BT was 3308.00±271.33 seconds and 3758.00±184.53 seconds for the mice treated with high dose total diterpenoids extracted with heating reflux and cold soak method,respectively,and significantly longer than that of blank control（2094.00±58.59 seconds）.Conclusion Total diterpenoids extrated from Isodon japonica（Burm.f.） Hara var.glaucocalyx（Maxim.） Hara could extend capillary blood coagulation time and tail bleeding time and has anticoagulant effect in mice.

关 键 词：蓝萼香茶菜总二萜 凝血时间 出血时间 

分 类 号：R181.23[医药卫生—流行病学]