高效毛细管电泳法测定夏枯草中4种活性成分的含量  

Four Active Component Assay of Xiakucao with High Performance Capillary Electrophoresis

作　　者：郭兴辉[1] 王倩雯[2] 刘伟[3] 

机构地区：[1]河南省食品药品检验所,河南郑州450000 [2]中山大学药学院,广东广州516000 [3]河南中医学院,河南郑州450008

出　　处：《中医学报》2013年第1期69-71,共3页Acta Chinese Medicine

基　　金：河南省教育厅自然科学研究计划项目(2009A360010)

摘　　要：目的:建立高效毛细管电泳法测定夏枯草中4种活性成分齐墩果酸、熊果酸、迷迭香酸和咖啡酸的含量。方法:采用胶束毛细管电泳法测定齐墩果酸、熊果酸的含量;毛细管区带电泳法测定迷迭香酸和咖啡酸的含量。结果:齐墩果酸、熊果酸、迷迭香酸、咖啡酸在54.8～657.6 mg.L-1、102.4～819.2 mg.L-1、20.4～163.2 mg.L-1、10.2～81.6 mg.L-1内线性关系良好;r分别为0.999 8、0.999 9、0.999 4、0.999 2;平均回收率为95.8%、95.6%、97.7%、96.7%;RSD为1.9%、1.9%、1.6%、2.0%。结论:该方法简便、快速、准确并且专属性强,可用于测定夏枯草药材中齐墩果酸、熊果酸、迷迭香酸和咖啡酸4种活性成分的含量。Objective：To establish a high performance capillary electrophoresis（HPCE） method to determine the content of oleanolic acid,ursolic acid,rosmarinci acid and caffeic acid in Xiakucao.Methods：To determine the content of oleanolic acid and ursolic acid using micellar capillary electric method.Determine the content of rosmarinci acid and caffeic acid using capillary zone electrophoresis.Results：The content of oleanolic acid,ursolic acid,rosmarinci acid and caffeic acid had good linear relation in 54.8-657.6 mg·L-1,102.4-819.2 mg·L-1,20.4-163.2 mg·L-1 and 10.2-81.6 mg·L-1.R was separately 0.999 8,0.999 9,0.999 4 and 0.999 2.The average recovery was 95.8%,95.6%,97.7% and 96.7%.RSD was 1.9%,1.9%,1.6% and 2.0%.Conclusion：This method was simple,rapid,accurate with a strong specificity and can be used as a determination of the content of oleanolic acid,ursolic acid,rosmarinci acid and caffeic acid in Xiakucao.

关 键 词：高效毛细管电泳法 夏枯草 齐墩果酸 熊果酸 迷迭香酸 咖啡酸 

分 类 号：R284.2[医药卫生—中药学]