股骨头坏死愈颗粒的质量评价方法研究  

Quality Rating of Gugutouhuaisiyu Granules

作　　者：杜天信[1,2] 张毅[2] 杜志谦 周凌[2] 李洁 罗石任[2] 王秀真[2] 

机构地区：[1]河南省正骨研究院,河南洛阳471000 [2]河南中医学院,河南郑州450046

出　　处：《中医学报》2013年第1期82-84,共3页Acta Chinese Medicine

摘　　要：目的:建立股骨头坏死愈颗粒的质量评价方法。方法:用薄层色谱法对股骨头坏死愈颗粒中的杜仲、鸡血藤、补骨脂进行定性鉴别;用高效液相色谱法测定股骨头坏死愈颗粒中蜕皮甾酮的含量。结果:薄层色谱鉴别法斑点清晰,分离度好,且阴性无干扰。含量测定中蜕皮甾酮在0.064 0～0.320 0 g.L-1范围内呈良好的线性关系,r=0.999 6,平均加样回收率为97.71%(n=6)。结论:该方法简单易行,科学合理,可用于股骨头坏死愈颗粒的质量控制。Objective：To establish the quality rating of Gugutouhuaisiyu granules.Methods：To use thin layer chromatography to make qualitation identification of Duzhong,Jixueteng and Buguzhi in Gugutouhuaisiyu granules.Use high performance liquid chromatography to determine the ecdysterone content.Results：The spots were clear with good resolution and non-interference negative.The content determination of ecdysterone had a good linear relationship in 0.064 0-0.320 0 g·L-1 range,r=0.999 6.The average sample recovery was 97.71%（n=6）.Conclusion：This method is simple,scientific and reasonable and can be used as quality control of the Gugutouhuaisiyu granules.

关 键 词：股骨头坏死愈颗粒 药物质量评价 高效液相色谱法 薄层色谱法 蜕皮甾酮 

分 类 号：R284.2[医药卫生—中药学]