两台Sysmex UF-1000i全自动尿沉渣分析仪检测结果的比对研究  

Comparative analysis of two Sysmex UF-1000i automated urinary sediment analyzers

作　　者：熊志刚[1] 黄小天[1] 余洋[2] 曾瑾忱[2] 

机构地区：[1]四川省医学科学院/四川省人民医院检验科,成都610072 [2]川北医学院检验系,2007级四川南充637000

出　　处：《检验医学与临床》2013年第1期21-22,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨实验室内不同全自动尿沉渣分析仪检测结果之间的差异是否在允许范围之内,以提高检验结果的准确性、一致性、可比性。方法以Sysmex UF-1000i全自动尿沉渣分析仪(LJ-25)作为标准仪器,在两台全自动尿沉渣分析仪均处于正常状态下,双份检测新鲜尿液,获取红细胞计数(RBC/μL)、白细胞计数(WBC/μL)、上皮细胞计数(EC/μL)、管型计数(CAST/μL)及细菌计数(BACT/μL),将检测结果进行通过率计算及比对试验,并参照Sysmex公司的仪器说明书提供的标准作为各参数的允许范围进行评估。结果两仪器检测结果显示,RBC、WBC、EC、CAST及BACT比对通过率88.09%、88.09%、92.86%、52.38%、35.71%;线性相关系数(r)为0.998、0.997、0.993、0.417、0.971,CAST、BACT结果比较差异无统计学意义(P>0.05),RBC、WBC、EC结果比较差异有统计学意义(P<0.05)。结论临床实验室应加强对所有检测项目结果的可靠性进行验证,做好尿沉渣分析仪室内质控的同时定期进行仪器比对试验,以保证不同仪器上的检测结果具有可比性、准确性、一致性、持续性的质量保证。Objective To compare the test results from the different Sysmex UF-1000i automated urinary sediment analyzers,and to explore whether the variance of the results were within the permitted range for improving the accuracy and consistency.Methods Under the condition that two urinary sediment analyzers were normal,two fresh urine samples were examined in duplicate,and the test data of RBC/μL、WBC/μL、EC/μL、CAST/μL and BACT/μL were compared with the results determined by traceable urinary sediment analyzer,and regression equations were established,then the passing rate was counted and test results were evaluated with the reference provided by SYSMEX company.Results The verification analyzers and the reference analyzer showed that RBC,WBC,EC,CAST and BACT passing rates were 88.09%,88.09%,92.86%,52.38%,35.71%,coefficients of correlation（r） exceeded 0.998,0.997,0.993,0.417,0.971,and there was no statistical significance of CAST and BACT between two analyzers（P0.05）,but statistical differences of RBC,WBC and EC were observed（P0.05）.Conclusion Clinical laboratory should strengthen the reliability of all project detection results for verification,regular comparison test and complete the urinary sediment analyzers interior quality control for ensuring test results of different instruments comparable,accuracy and consistency.

关 键 词：UF-1000i全自动尿沉渣分析仪 比对试验 红细胞 白细胞 管型 

分 类 号：R446.12[医药卫生—诊断学]