男性不育伴乙型肝炎病毒感染患者精子DNA完整性的初步研究  

Sperm DNA integrity of infertile males with hepatitis B virus infection

作　　者：邓天勤[1,2] 黄永汉[1] 甄劲仪 卢建棠[1] 李颖嫦[1] 谭兴银[1] 刘雄春[1] 阮建波[2] 张海滨[1] 

机构地区：[1]佛山市第一人民医院泌尿外科,广东佛山528000 [2]东莞市太平人民医院男科,广东东莞523901 [3]佛山市南海区人民医院妇产科,广东佛山528200

出　　处：《中华男科学杂志》2013年第1期72-76,共5页National Journal of Andrology

基　　金：广东省医学科研基金(A2010600)~~

摘　　要：目的:探讨男性不育伴乙型肝炎病毒感染患者精子DNA完整性。方法:应用精子染色质结构分析检测90例男性不育伴乙型肝炎病毒感染者(A组)、82例男性不育者(B组)及70例正常生育者(C组)的精子DNA完整性:DNA碎片化指数(DFI)和高DNA可染性(HDS),分别比较3组之间精子DNA完整性的差异。结果:精子DNA完整性:DFI:A组为(28.17±13.06)%,高于B组(26.64±9.79)%和C组(15.67±4.73)%,A组及B组分别与C组比较差异有统计学意义(P<0.05),A组与B组比较差异无统计学意义(P>0.05);HDS:A组为(10.83±5.60)%,高于B组(9.04±3.48)%及C组(8.04±2.25)%,A组与C组比较差异有统计学意义(P<0.05)。结论:男性不育患者的精子DNA完整性与正常生育者存在差异,若伴乙型肝炎病毒感染,会进一步加重精子DNA损伤,主要表现为精子核成熟异常。[ Abstract] Objective： To investigate sperm DNA integrity in male infertility patients with hepatitis B virus （HBV） infection. Methods： This study included 90 infertile men with HBV infection （group A）, 82 infertile men without HBV infection （group B） and 70 normal fertile men （group C）. We detected sperm DNA integrity among the subjects, including DNA fragmentation index （DFI） and high DNA stainability （HDS）, by sperm chromatin structure assay （SCSA） , and compared them among the three groups. Re- sults ： DFI was higher in group A （ [ 28.17 ± 13.06 ] % ） than in B （ [ 26.64 ±9.79 ] % ） and C （ [ 15.67 ±4.73 ] % ）, significantly higher in A and B than in C （ P 〈 0.05 ） but with no significant difference between A and B （ P 〉 O. 05 ）. HDS was higher in group A （ [10.83± 5.60 ] % ） than in B （[ 9.04± 3.48 ] % ） and C （ [8.04 ± 2.25 ] % ）, with significant difference between A and C （ P 〈 0.05 ）. Conclusion ： Sperm DNA integrity of infertile males is significantly different from that of normal fertile men, and infertilitywith HBV infection further impairs sperm DNA, which is manifested by abnormal sperm nuclear maturity.

关 键 词：乙型肝炎病毒 男性不育 

分 类 号：R698.2[医药卫生—泌尿科学] R512.62[医药卫生—外科学]