人参锈腐病菌产孢条件研究  

Sporulation conditions of Cylindrocarpon destructans

作　　者：贾斌[1] 金海强[1] 赵贞丽[2] 李熙英[1] 

机构地区：[1]延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002 [2]延边大学农学院,吉林延吉133002

出　　处：《中国森林病虫》2013年第1期1-3,23,共4页Forest Pest and Disease

基　　金：吉林省教育厅重点项目(2011800-611010002);延边大学科技发展计划项目(200802)

摘　　要：为了探明人参锈腐病菌Cylindrocarpon destructans(Zinss)Scholten的产孢条件,在室内不同培养条件下对人参锈腐病菌产孢条件进行了研究。结果表明,病菌分生孢子产生的适宜温度为15～25℃;厚垣孢子产生的适宜温度为10～20℃,最适为15℃。产生分生孢子和厚垣孢子的适宜pH值为5～7,最适pH值为6。不同碳源上病菌均能产生分生孢子和厚垣孢子,其中葡萄糖和果糖对分生孢子的产生促进最大,但不同的碳源对厚垣孢子产量的影响不明显。分生孢子在尿素为氮源的培养基上大量产生,在缺氮培养基上不产生;厚垣孢子在不同氮源培养基上均能产生,在酵母膏培养基上产量最大。不同培养基中,在人参根培养基上分生孢子产生所需时间短,产量高;厚垣孢子在PDA和基础培养基上产生所需时间短,产量高。光照条件对分生孢子的产生没有明显的影响,但对厚垣孢子的产生有明显的抑制作用。In order to find out the conditions for sporulation of Cylindrocarpon destructans,the investigation of sporulation of C.destructans under different culture conditions was conducted.The results showed that the suitable temperature for sporulation of conidia ranged from 15 ℃ to 25 ℃ whereas the suitable temperature for sporulation of chlamydospores ranged from 10 ℃ to 20 ℃ with the optimum temperature of 15 ℃.The suitable pH value for conidia and chlamydospores was varied from 5 to 7 with the optimum pH of 6.All the pathogens cultured on various carbon source medium could produce conidia and chlamydospores.Among the carbon sources,glucose and fructose were better for producing conidia.Nevertheless,no obvious influence of carbon sources on chlamydospores production was observed.The pathogens could produce a greater amount of conidia on carbamide medium,but did not produce conidia on nitrogen deficient medium.All the pathogens cultured on different nitrogen source medium could produce chlamydospores;among these,the pathogens cultured on the yeast extract medium produced the highest amount of chalmydospores.The greatest amount of conidia was produced in less time when the pathogens was cultured on ginseng root medium,whereas a greater amount of chlamydospores was produced in less time when the pathogens was cultured on PDA and basic medium.Light conditions had not evident influence on conidia production,but had an obvious inhibition effect on chlamydospores production.

关 键 词：人参锈腐病菌 菌丝 分生孢子 厚垣孢子 产孢条件 

分 类 号：S763.15[农业科学—森林保护学]