柠檬酸镧对人肺癌PG细胞、人胃癌BGC-823细胞的作用  

Effect of Lanthanum Citrate on Two Cell Lines: Human Lung Cancer Cells PG and Human Gastric Carcinoma Cells BGC-823

作　　者：李晓滨[1] 周爱儒[1] 俞文华 陈兴安 

机构地区：[1]北京医科大学生化与分子生物学系,北京100083 [2]国家稀土开发应用卫生评价中心,北京100088

出　　处：《中国稀土学报》2000年第2期156-159,共4页Journal of the Chinese Society of Rare Earths

摘　　要：采用噻唑蓝 (MTT)测定、3H TdR参入、流式细胞术、荧光偏振技术和软琼脂培养 ,观察了柠檬酸镧 (LaCit)对人肺癌细胞 (PG)、人胃癌细胞 (BGC 82 3 )的增殖、DNA合成、细胞周期、细胞膜脂流动性、软琼脂生长的影响。LaCit的浓度为 0 0 5 ,0 1,0 5 ,1mmol·L- 1 时可抑制肺癌细胞PG的增殖 :浓度为 0 5 ,1mmol·L- 1 时可抑制肺癌细胞PG ,DNA的合成 ,其G1 期细胞比例增加 ,S期细胞比例减少 ,细胞膜脂流动性降低 ,在软琼脂中的生长能力降低。而同样浓度的LaCit对胃癌细胞BGC 82 3除可降低其细胞膜脂流动性外 ,未见其他影响。The effect of LaCit on cell proliferation, DNA synthesize, cell cycle, cell membrane fluidity, growth in soft agar in human lung cancer cells PG and human gastric carcinoma cells BGC 823 was studied by MTT(3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide) assay, 3H TdR incorporation, flow cytometry, fluorescence polarization assay, and soft agar culture. The results indicate that LaCit inhibits PG cells proliferation when the concentrations are 0 05, 0 1, 0 5, and 1 mmol·L -1 . When the concentrations are 0 5 and 1 mmol·L -1 , LaCit inhibits PG cells 3 H TdR incorporation, and decreases the proportion of PG cells in S phase while increases the proportion of PG cells in G 1 phase and decreases the membrane fluidity of PG cells. But LaCit has no effect on BGC 823 cells except cell membrane fluidity and no effect on 2BS cells.

关 键 词：稀土 柠檬酸镧 PG细胞 BGC-823细胞 肺癌 胃癌 

分 类 号：O614.33[理学—无机化学] R318.04[理学—化学]