犬盆底疝动物模型的建立  

Establishment of dog pelvic floor hernia model

作　　者：杨硕[1] 陈杰[1] 申英末[1] 刘素君[1] 王明刚[1] 田茂霖[1] 

机构地区：[1]首都医科大学附属北京朝阳医院疝和腹壁外科,北京100043

出　　处：《中华实验外科杂志》2013年第1期163-164,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81041014）

摘　　要：目的探讨以实验犬为平台建立盆底疝的动物模型的可行性。方法入组8条犬，通过外科操作打开犬盆底肌肉筋膜组织间隙（肛门外括约肌、坐骨尾骨肌和肛提肌、闭孔内肌间的间隙），再通过围手术期诱导便秘提高实验犬腹内压以制备犬盆底疝的动物模型，解剖并观察实验犬盆底疝的发生，以验证盆底疝动物模型的成功建立。结果8条犬术后1周伤口均愈合，3例术后出现局部红肿，2周后消退，8条犬术后1个月内（最短术后3d，最长术后18d）均出现盆底膨出，解剖操作证实盆底疝模型制备成功。结论该方法制备犬盆底疝模型的方法简单、易行。Objective To explore the feasibility of establishing animal model of pelvic floor hernia by experimental dog. Methods Eight dogs involved in the experiment, and the pelvic floor muscle fascia tissue space among external anal sphincter, ischiococcygeus, levator ani muscle and internal obturator muscle was dissected by surgical operation. Meanwhile, constipation was induced peri-operatively to increase intra-abdominal pressure for establishing animal model of pelvic floor hernia in experimental dogs. Successful establishment of animal model of pelvic floor hernia was validated by observing and dissecting the pelvic floor hernia in experimental dogs. Results All of the 8 dogs＇ operative incisions achieved primary healing in 1 week, 3 dogs had local red swelling of the skin and disappeared in 2 weeks, and all pelvic floor prolapse appeared in 1 month after operation （3rd day to 18th day）. Successful establishment of animal model of pelvic floor hernia was validated by the operation of anatomy. Conclusion The method explored above to establish animal model of pelvic floor hernia by experimental dog is simple and feasible.

关 键 词：盆底疝 模型 动物 犬 

分 类 号：R7[医药卫生—临床医学] R-332