疏肝健脾、安神和胃法治疗功能性腹泻模型大鼠的作用机制  

Effect of the principle for soothing the liver and strengthening the spleen,regulating stomach and refresh spirit on corticotropin releasing hormone content of functional diarrhoea rats

作　　者：吴文江[1] 陶双友[2] 韩棉梅[2] 梁嘉恺[2] 罗琦[2] 何丽英[2] 周福生[2] 

机构地区：[1]广东省中医院,广东广州510120 [2]广州中医药大学脾胃研究所

出　　处：《中国老年学杂志》2013年第3期577-579,共3页Chinese Journal of Gerontology

基　　金：国家973计划项目(No.2009CB523002)

摘　　要：目的基于心胃相关理论探讨母子分离加直肠刺激法导致功能性腹泻的发病机制来探讨中药疏肝健脾、安神和胃法治疗腹泻的作用途径。方法采用母子分离加直肠刺激法复制大鼠腹泻模型,并显微镜下观察腹泻模型大鼠肠黏膜是否出现器质性改变。给予疏肝健脾、安神和胃中药2 w后观察大鼠小肠推进率;并采用放射免疫法检测大鼠下丘脑、脑干以及肠黏膜的促肾上腺激素释放激素水平及中药中药疏肝健脾、安神和胃法对其影响。结果造模后大鼠小肠推进率较正常组明显增快(P<0.05)。经中药疏肝健脾、安神和胃法治疗2 w后,大鼠小肠推进较模型组降低(P<0.05),与正常组比较无显著性差异。与正常相比,造模后大鼠脑干、下丘脑及肠黏膜促肾上腺激素释放激素含量明显升高(P<0.05);给予疏肝健脾、安神和胃中药2 w,治疗后大鼠脑干、下丘脑及肠黏膜促肾上腺激素释放激素水平与腹泻模型组大鼠相比均有所降低(P<0.05),与正常组及西药对照组比较无显著性差异。结论腹泻模型大鼠脑干、下丘脑及肠黏膜促肾上腺激素释放激素含量表达的升高提示其表达改变与腹泻的发病相关。中药疏肝健脾、安神和胃能降低腹泻大鼠脑干、下丘脑及肠黏膜促肾上腺激素释放激素含量,其作用机制可能是与影响脑肠轴促肾上腺激素释放激素通路有关。Objective To find the mechanism of functional diarrhoea through this animal experiments, which based on the heart and stomach theory, and try to prove the effect of principle for soothing the liver and strengthening the spleen. Methods A rat model of functional diarrhoea was established by separation of mothers with children and rectal stimulation in young rats. After the treatment of the principle for soothing the liver and strengthening the spleen, regulating stomach and refresh spirit for two weeks, the corticotropin releasing hormone level expression was tested. Results After 2 weeks of the treatment, intestinal propulsion rate of functional diarrhoea rats was significantly reduced （ P 〈 0. 05 ）. Compared with the normal group, the corticotropin releasing hormone level of model group was significantly increased （ P 〈 0. 05 ）. Conclusions The eorticotropin releasing hormone expression levels in brain stem, hypothalamus, intestinal mucosa of functional diarrhoea rat are often associated with the pathogenesis of functional diarrhoea. The principle for soothing the liver and strengthening the spleen, regulating stomach and refresh spirit lower the expression of corticotropin releasing hormone explains the pathogenesis of functional diarrhoea.

关 键 词：心胃相关理论 功能性腹泻 促.肾上腺激素释放激素 

分 类 号：R55[医药卫生—血液循环系统疾病]