大蜡螟中类肽聚糖识别蛋白Gm21的鉴定、基因克隆及序列分析  

Identification,cDNA cloning and sequence anlysis of a peptidoglycan-recognition-like protein Gm21 in Galleria mellonella

作　　者：石怀兴[1] 曾洪梅[1] 邱德文[1] 

机构地区：[1]中国农业科学院植物保护研究所农业部有害生物综合治理重点实验室,北京100081

出　　处：《应用昆虫学报》2013年第1期118-125,共8页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金(31071741)

摘　　要：为了调查毒素蛋白免疫下大蜡螟Galleria mellonella L.产生的免疫相关蛋白,采用双向电泳的方法,从大蜡螟血淋巴中鉴定得到一种昆虫肽聚糖识别蛋白Gm21,通过RT-PCR、cDNA末端快速扩增(RACE)技术克隆得到其全长基因,该基因全长cDNA具有完整的编码框,编码211个氨基酸,序列分析表明该蛋白是一种具有潜在酰胺酶活性的S型肽聚糖识别蛋白。本研究中Gm21蛋白在免疫压力下的上调表达及其cDNA序列的克隆,对进一步深入研究昆虫肽聚糖识别蛋白的功能具有重要的意义。An investigation was made of the immune protein in Galleria melloneUa L. that had been immune-challenged with toxic protein. A peptidoglycan-recognition protein, Gm21, in hemolymph of G. mellonella was identified by two dimensional （2-D） electrophoresis, and the encoding gene was cloned by RT-PCR and RACE. The full length cDNA has the complete open reading frame and encodes a protein of 211 amino acids. Sequence analysis indicated that the protein is a peptidoglycan-recognition protein （PGRP） -S-like protein with potential amindase activity. The increased expression of Gm21 protein in immune-challenged G. mellonella and molecular cloning of the Gm21 gene are important techniques for further study of peptidoglycan-recognition proteins.

关 键 词：大蜡螟 肽聚糖识别蛋白 免疫 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]