抗Cry1Ac小菜蛾种群生命表及其适合度研究  

Life table and relative fitness of a strain Plutella xylostella resistant to Bacillus thuringiensis Cry1Ac toxin

作　　者：秦悦[1] 朱勋[1] 雷妍圆[1] 郑晓旭[1] 吴青君[1] 王少丽[1] 张友军[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《应用昆虫学报》2013年第1期154-160,共7页Chinese Journal of Applied Entomology

基　　金：国家科技支撑课题(2012BAD19B06);公益性(农业)行业科技专项(201103021)

摘　　要：本实验研究了小菜蛾Plutella xylostella L.高抗Cry1Ac种群DBM1Ac-R(抗性倍数大于1000)及敏感种群DBM1Ac-S的生长发育、繁殖等生物特征情况,组建了两种群的生命表,并对其适合度进行了研究。结果表明,DBM1Ac-R的产卵量、卵历期、孵化率和化蛹率、蛹重及雌雄比(♀:♂),均显著低于DBM1Ac-S。DBM1Ac-R相对于DBM1Ac-S的相对适合度为0.5762,表明Cry1Ac抗性种群在繁殖能力上存在明显的生存劣势。A resistant strain （DBM1Ac-R） of Plutella xylostella L. （diamondback moth） with more than 1 000-fold resistance to CrylAc was obtained. Life history characteristics, such as the development and fecundity of susceptible （DBM1Ac-S） and resistant strains, were studied. Life tables were established and the relative fitness cost of developing resistance analyzed. The results show that the DBM1Ac-R strain has distinct reproductive disadvantages, such as a fitness value of 0. 5762, including lower fecundity, hatching rate, pupation rate and sex ratio （ 9 ：δ ） , shorter egg period and lighter pupa weight, compared with the non-resistant DBM1Ac-S strain.

关 键 词：小菜蛾 Cry1Ac毒蛋白 生命表 相对适合度 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]