姜黄素对肿瘤坏死因子-α诱导的HaCaT细胞增殖及凋亡的影响  

Effect of curcumin on the proliferation of and apoptosis in HaCaT cells induced by tumor necrosis factor a

作　　者：杨晓红[1] 曹毅[1] 刘改荣[1] 代群[1] 解凡[1] 李园园[1] 陈微[1] 

机构地区：[1]浙江中医药大学附属第一医院,杭州310006

出　　处：《中华皮肤科杂志》2013年第2期84-87,共4页Chinese Journal of Dermatology

基　　金：浙江省自然科学基金（Y2100759）

摘　　要：目的探讨姜黄素对肿瘤坏死因子（TNF）-α诱导的HacaT细胞增殖及凋亡的影响。方法MTT法检测不同浓度重组TNF-α（0、1、5、10、25、50、100ng／m1）、姜黄素20μmol／L对Hacarr细胞增殖的影响。蛋白印迹实验检测25ng／ml TNF-α和20μmol/L姜黄素作用下Hacarr细胞PCNA、Notch-1的蛋白表达。使用Annexin-V/PI试剂盒在流式细胞仪上检测25ng／mlTNF—d和20μmol／L姜黄素作用下HacaT细胞早期凋亡情况。结果。TNF-α呈浓度依赖性促进HacaT细胞增殖，25ng／mlTNF-α对HacaT细胞的促增殖基本达到最大，20μmol／L姜黄素有效抑制25ng／ml TNF-α对HacaT细胞的促增殖作用，25ng／mlTNF-α对HaCaT细胞无明显促凋亡作用，而20μmol／L姜黄素有效促进HaCaT细胞发生凋亡，并可激发25ng／ml TNF-α对HaCaT细胞的凋亡诱导作用。结论姜黄素可激发TNF-α对HaCaT细胞的促凋亡作用，同时抑制TNF-α对HaCaT细胞促增殖作用。Objective To evaluate the effect of curcumin on the proliferation of and apoptosis in HaCaT cells induced by tumor necrosis factor ot （TNF-a）. Methods HaCaT cells were cultured with the presence of different concentrations （0, 1, 5, 10, 25, 50, 100 ng/ml） of recombinant TNF-a, cureumin of 20 μmol/L, or the combination of recombinant TNF-a （25 ng/ml） and cureumin （20 μmol/L）, for 24 hours followed by the determination of cell proliferation with methyl thiazolyl tetrazolium （MTT） assay. Western blot was conducted to measure the protein expression of proliferating cell nuclear antigen （PCNA） and Notch-1 in HaCaT cells treated with recombinant TNF-a （25 ng/ml） and curcumin （20 μmol/L） alone or in combination for 24 hours. Flow cytometry using annexin-V/propidium iodine （PI） was performed to assess the early apoptosis in HaCaT cells incubated with recombinant TNF-a of 25 ng/ml and curcumin of 20 μmol/L alone or in combination for 12 hours. Statistical analysis was carried out with one-way analysis of variance. Results Recombinant TNF-a promoted the proliferation of HaCaT cells in a dose-dependent manner, with the maximum proliferation activity observed in HaCaT cells treated with TNF-a of 25 ng/ml, while cureumin of 20 μmol/L effectively inhibited the proliferation of HaCaT cells induced by TNF-a of 25 ng/ml （P 〈 0.01）. TNF-a of 25 ng/ml had no obvious effect on cell apoptosis, while curcumin of 20 μmol/L markedly induced the apoptosis in HaCaT cells, and there was a synergy between TNF-a of 25 ng/ml and cureumin of 20 μmol/L in the induction of apoptosis in HaCaT ceils, with the apoptosis rate being 2.3%, 3.4%, 11.6% and 16.8% respectively in untreated cells, cells treated with TNF-a, curcumin, and the combination of TNF-a and cureumin, respectively. Conclusions Curcumin could enhance the inductive effect of TNF-a on the apoptosis in, but suppress the promotive effect of TNF-a on the proliferation of, HaCaT cells.

关 键 词：银屑病 肿瘤坏死因子-Α 姜黄素 

分 类 号：R285.5[医药卫生—中药学]