瘦素抗体对兔耳增生性瘢痕组织转化生长因子-β1mRNA表达的影响  

Effects of leptin antibody on the expression of transforming growth factor-β1 mRNA in hypertrophic scarmodel in rabbit ears

作　　者：安英华[1] 金英姬[1] 方宇辉[1] 金哲虎[1] 

机构地区：[1]延边大学附属医院皮肤科,吉林延吉133000

出　　处：《中华皮肤科杂志》2013年第2期109-112,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81060233）,吉林省教育厅[吉教科合字（2012）第2号],吉林省卫生厅（20112085）

摘　　要：目的探讨瘦素抗体对兔耳增生性瘢痕组织内转化生长因子（TGF）-β1mRNA表达的影响。方法新西兰大白兔15只，每只大白兔双耳腹侧作直径约7mm，深达软骨膜的圆形切口共6个，共获得兔耳增生性瘢痕模型90个，随机分为3组。生理氯化钠液组：生理氯化钠液外用40d；瘦素抗体外用组：2ng／ml瘦素抗体外用40d；瘦素抗体外用＋注射组（联合用药组）：2ng／ml瘦素抗体外用14d后，每周注射1次2ng／ml瘦素抗体，共3次。术后第40天各组均取瘢痕组织，计算瘢痕增生指数、组织病理学检查及测定瘢痕组织内TGF-β1mRNA表达。结果瘦素抗体外用组和联合用药组瘢痕增生指数及瘢痕组织中TGF-β1mRNA的表达明显下降，与生理氯化钠液组相比较，差异具有统计学意义，但两组之间比较，差异无统计学意义。组织病理学表明，生理氯化钠液组毛细血管增生明显，有大量成纤维细胞，核大、密集，排列不规则；瘦素抗体外用组与联合用药组成纤维细胞数量明显较少，核较小，排列相对规则。结论瘦素抗体治疗能降低兔耳增生性瘢痕组织内的TGF-β1mRNA表达。Objective To estimate the effect of leptin antibody on transforming growth factor （TGF）-β1 mRNA expression in hypertrophic scar model in rabbit ears. Methods Fifteen New Zealand white rabbits were included in this study. Three circular incisions which measured 7 mm in diameter and reached the perichondrium, were made in each ear of these rabbits to establish 90 models of hypertrophic scar. After the operation, these models were randomly and equally divided into 3 groups to be treated with topical sodium chloride physiological solution for 40 days （saline group）, topical leptin antibody of 2 ng/ml for 40 days （leptin antibody group）, and topical leptin antibody of 2 ng/ml for 14 days followed by injection of leptin antibody of 2 ng/ml once a week for 3 weeks （combination group）. Scar tissue was resected from these rabbit ears at 40 days after the operation, followed by the determination of scar elevation index, histopathological examination by using haematoxylin and eosin staining, and quantification of TGF-β1 mRNA expression by real-time fluorescence-based PCR. SPSS 13.0 software was used for data processing. Statistical analysis was carried out by one-way analysis of variance. Results A significant decrease was observed in scar elevation index （2.33 ± 0.33 and 2.35± 0.22 vs. 3.33 ± 0.41, both P 〈 0.05） and TGF-β1 mRNA expression in the leptin antibody group and combination group compared with the control group, whereas no significant difference was observed between the leptin antibody group and combination group in either of the two parameters. Pathologically, there was an apparent proliferation of capillaries in the saline group with numerous irregularly and densely arranged fibroblasts with large nuclei, while relatively few fibroblasts with small nuclei, which were arranged in a more regular way, were observed in the leptin antibody group and combination group. Conclusion Leptin antibody treatment can reduce the expression of TGF-β1 mRNA in hypertrophic scar tissue in rabbit ears.

关 键 词：瘢痕 瘦素 抗体 转化生长因子Β1 兔 

分 类 号：R6[医药卫生—外科学]