黄芩甙对大鼠脑胶质瘤的抑制作用  

Anfitumor effect of baicalin on rat brain glioma

作　　者：胡永珍[1] 王殿洪[1] 栾彧 龚海东[1] 

机构地区：[1]哈尔滨医科大学附属第一医院神经外科,150001 [2]哈尔滨第一医院核磁共振室

出　　处：《中华肿瘤杂志》2013年第1期11-16,共6页Chinese Journal of Oncology

基　　金：黑龙江省中医管理局基金项目（ZHY08-274）;哈尔滨医科大学附属第一医院科研基金（2007049）

摘　　要：目的研究黄芩甙对大鼠脑胶质瘤的作用机制。方法建立Wistar大鼠脑深部胶质瘤模型。接种c6胶质瘤细胞后7d，将大鼠随机分为对照组（0．9％氯化钠注射液30nag·kg-1·d-1，灌胃）、小剂量组（黄芩甙50nag·kg-1·d-1，灌胃）、中剂量组（黄芩甙100mg·kg-1·d-1，灌胃）和大剂量组（黄芩甙200mg·kg-1·d-1，灌胃）。分析各组大鼠治疗后脑胶质瘤的病理学、MRI影像学和电镜检查结果，采用免疫组化染色检测p53和Bcl-2的表达，分析大鼠的平均生存时间和体重变化情况。结果与对照组比较，大剂量组大鼠处死（濒死）前的肿瘤体积为（343．62±28．98）mm3，显著缩小（P〈0．01），生存时间为（42．83±4．77）d，显著延长（P〈0．01）。对照组胶质瘤细胞p53蛋白强阳性细胞百分比为（84．47±3．74）％，中度阳性细胞百分比为（47．28±2．38）％，与阴性组[（12．91±1．07）％]比较，差异均有统计学意义（均P〈0．01）；大剂量组p53蛋白强阳性细胞百分比与对照组比较，差异有统计学意义（P〈0．01）。对照组Bcl-2蛋白强阳性细胞百分比为（86．51±4．17）％，中度阳性细胞百分比为（48．19±2．11）％，与阴性组[（10．36±1．43）％]比较，差异均有统计学意义（均P〈0．01）。电镜检查结果显示，黄芩甙可使胶质瘤细胞核和细胞器发生破坏。结论黄芩甙对在体脑胶质瘤有明显抑制作用，其作用机制可能与下凋突变型p53引发细胞凋亡有关，但与Bcl-2无关。Objective To investigate the therapeutic mechanism of baicalin on rat brain glioma. Methods Deep brain glioma models were established by injection of glioma cell line C6 cells into the brain of Wistar rats. The rats at 7 days after modeling were randomly divided into tumor control group（0.9% NaC1 solution 30 mg · kg-1 · d-1 gavage）and experimental groups. The experimental rats was divided into 3 groups： low dose group （ 50 mg · kg-1 · d-1 ）, middle dose group （ 100 mg ·kg-1 ·d-1 ） and high dose group （ 200 mg· kg-1 · d-1 ）, given the baicalin by gavage. Pathological and electron microscopic changes were observed. The expressions of p53 and Bcl-2 were determined by immunohistochemistry, and the changes of MR_I, the average survival time and body weight of the rats in each group after treatments were analyzed. Results Compared with the control group, the tumor diameter and volume of high dose group rats before sacrifice were significantly reduced （ P 〈 0.01 ）, and the survival time was significantly prolonged （P 〈 O. O1 ）. Immunohistochemistry revealed strong positive expression rate of mutant p53 （84. 47 ± 3.74） % and moderately positive rate （47.28 ± 2.38）% in the control group, significantly higher than that in the negative group （ 12.91± 1.07 ） % （ P 〈 0.01 ）. The positive rate of mutant p53 of the high dose group was （46.42 ±2.19）%, significantly lower than that of the control group （84.47 ±3.74）% （P 〈 0. 01 ）. The expression rate of Bcl-2 in the control group was strongly positive （86.51± 4. 17 ）% and moderate positive （48.19 ±2.11 ） %, signifieandy higher than that of the negative group （ 10.36 ± 1.43 ） % （ P 〈 0. 01 ）. Electron microscopy revealed that baiealin eansed damages of the cell nuclei and organelles in the gliornas. Conclusions Baiealin has significant inhibitory effect on glioma in vivo, and its mechanism may be related to cell apoptosis induced by down-regulated expression of mutant p53, but not relat

关 键 词：黄芩甙 脑胶质瘤 大鼠 P53 BCL-2 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R739.4[医药卫生—中医学]