全日制放牧BMY牛的活体采卵-体外胚胎生产-胚胎移植研究  

Ovum Pick up and in vitro Embryo Production and Embryo Transfer of BMY Beef Cattle under Whole Year Grazing System

作　　者：和占星[1] 王喆[1] 张继才[1] 王安奎[1] 金显栋[1] 袁希平[1] Joaquim M.Garcia Marina R.de Lima 何永珍[1] 黄必志[1] 

机构地区：[1]云南省草地动物科学研究院,昆明650212 [2]Department of Preventive Veterinary Medicine and Animal Reporduction,Faculty of Agricultural and Veterinary Sciences,Sao Paulo State University

出　　处：《畜牧兽医学报》2013年第1期38-48,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：云南省科技创新强省计划-国际科技合作专项(2008AC001);云南省技术创新人才培养项目(2010CI082);云南省农业科技创新工程(2008LA018);国家现代农业(肉牛)产业技术体系(CARS-038)

摘　　要：为建立BMY牛的活体采卵(OPU)-体外胚胎生产(IVP)-胚胎移植(ET)快速扩繁技术体系,从人工草地全放牧牛群中选择健康的经产空怀BMY牛作为供体(n=14)集中OPU一次;卵母细胞经TCM-199体外成熟培养(IVM)、Fert-TALP液体外受精(IVF),受精卵用合成输卵管液(SOF)体外培养(IVC)生产胚胎;BMY牛受体(n=81)用CUE-MATETM孕酮阴道栓+EB+FSH+PG方法同期发情处理,对黄体合格的移植OPU-IVP来源鲜胚。研究结果表明:①OPU头均获卵母细胞23.50枚,获10枚以上卵母细胞的供体占85.7%;IVP的平均囊胚数和可用胚数分别为5.21和4.00枚;OPU前8～9d卵巢上有黄体(CL)的供体,头均获卵母细胞数及IVP的囊胚数、可移植胚数、囊胚率和可移植胚率分别比对照组高7.28枚(P<0.05)、7.29枚(P<0.05)、6.00枚(P<0.05)、27.3%(P<0.01)和23.2%(P<0.01);②受体黄体合格率69.1%(56/81),胚胎移植妊娠率37.5%(21/56),产犊率35.7%(20/56);③受体在胚胎移植时注射GnRH,其妊娠率比对照组高16.2%(P>0.05)。结果显示,BMY母牛具有良好的OPU潜力,但个体间差异较大;可通过严格供体选择及改善IVP技术等措施,实现OPU-IVP效率最大化。CUE-MATETM+EB+FSH+PG同期发情处理方法适于在牛规模化胚胎移植(ET)和人工授精(AI)中应用。综合应用OPU、IVP、同期发情和ET技术是加快优良BMY牛扩繁的重要途径之一。The objective of this study was to establish a rapidly multiplication system of BMY cat- tle by the technology of ovum pick-up （OPU）-in vitro embryo production（IVP）-embryo transfer （ET）. 14 BMY cattle with non-pregnancy, healthy and multiparous were selected for OPU once under whole year grazing in the artificial grasslands. Oocytes were matured in vitro in TCM 199with 5% （v/v） fetal calf serum （FCS） and in vitro fertilization in Fert-TALP medium; zygotes were cultured in synthetic oviduct fluid （SOF） with 5o//oo （v/v） FCS for embryo production. 81 BMY recipients were picked out for estrus synchronization treatment using CUE-MATETM （Con- trolled intravaginal progesterone-release device） ＋ estradiol benzoate （EB） -t- follicle-stimulating hormone （FSH） ＋ prostaglandin F2oanalogue （PG）, and recipients with a well-developed CL were transferred by BMY fresh embryos derived from OPU-IVP. The result showed that： ① Average donors with OPU were obtained 23.50 oocytes, and 85.7% donors offered more than 10 oocytes. Meanwhile, average blastocysts and available embryos of IVP were 5. 21 and 4. 00, respectively; Average oocytes, blastocysts, transferable embryos, rates of blastocyst and availa- ble embryo of donor ovaries with CL before OPU for 8 or 9 days were 23.57, 8.86, 7.00, 37.6% and 29.7%, respectively, significantly higher than those of control group （15.29, 1.57, 1.00, 10.3% and 6.5%, respectively, P〈0.05 or 0.01）.②Ratios of corpus luteum （CL） up to A-B graded, pregnancy rate and calving rate were 69.1% （56/81）, 37.5% and 35.7%, respectively. ③ Pregnancy rates of recipients with injection GnRH at the time of ET was 46.2%, higher than that of control group （without GnRH） （30. 0%, P〉0. 05）. The results indicated that BMY cows had better potential for OPU, but there was significant difference among individual donors. Moreover, the maximal efficiency of OPU-IVP was obtained by strictly selecting donors and improving technology of IVP and othe

关 键 词：BMY牛 全日制放牧 超声波引导采卵 体外胚胎生产 胚胎移植 

分 类 号：S823[农业科学—畜牧学] S814.8[农业科学—畜牧兽医]