鸭Mef2bnb基因的克隆及其mRNA丰度在肌肉组织中的发育性表达变化  

Molecule Cloning and Developmental Expression of Mef2bnb Gene in Muscle Tissues from Duck

作　　者：王浩瀚[1] 刘贺贺[1] 孙玲利[1] 张荣萍[1] 李欣欣[1] 杨超[1] 李亮[1] 王继文[1] 

机构地区：[1]四川农业大学动物遗传育种研究所,温江611130

出　　处：《畜牧兽医学报》2013年第1期49-56,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家现代农业水禽产业技术体系(CARS-43-6)

摘　　要：旨在克隆鸭肌肉增强因子2b邻居基因(Myocyte enhancer factor 2Bneighbor,Mef2bnb)的cDNA全序列,并对其进行分析,研究该基因在肌肉组织中的发育性表达规律。利用RACE技术从鸭腿肌中克隆出Mef2bnb的全长cDNA序列,应用Q-PCR技术检测了自鸭胚胎10d至出壳1周肌肉组织中Mef2bnb基因的发育性表达。结果显示:该序列全长935bp,包括5′非编码区(5′UTR)51bp和3′非编码区(3′UTR)518bp,完全开放阅读框(ORF)366bp,可编码121个氨基酸残基,结构分析表明,该肽链没有信号肽,第1～117号氨基酸为鸭Mef2bnb基因与其它动物高度保守的NEP结构域;Mef2bnb基因在胸肌、腿肌和心脏3种肌肉组织中不同阶段均有不同程度的表达,而其在胸肌中的表达量均高于同时期在腿肌中的表达量,推测鸭Mef2bnb基因可能具有促进Ⅱ型纤维形成的功能,另外该基因在心脏的高表达表明其可能与心脏的发育有关。本研究结果将为进一步研究鸭Mef2bnb结构及其在肌肉组织中的作用奠定基础。This experiment was conducted to obtain and analyze cDNA sequence of duck Mef2bnb （Myocyte enhancer factor 2B neighbor）, and to investigate the developmental expression of Mef2bnb in duck muscle tissues. The full-length Mef2bnb cDNA was cloned from leg muscle of duck by using RACE method. Meanwhile, real-time quantitative PCR method was used to ana- lyze Mef2bnb gene developmental expression in muscle tissues of duck, which from embryo ten days to one week after birth. The results showed that full sequence of duck Mef2bnb gene was 935 base-pairs（bp） including a 5＇untranslated region（UTR） of 51 bp and 3＇UTR of 518 bp, a complete open reading frame （ORF）of 366 bp encoding 121 amino acid （aa） residues. The struc- tural analysis indicates that there was no signal peptide in this peptide chain, and the amino acid from I to 117 was a NEP domain which was conservative between the Mef2bnb of duck and other animals. Moreover, the different expression levels of Mef2bnb were found in chest muscle, leg muscle and heart tissue. The expression of Mef2bnb in chest muscle was higher than that in leg muscle at the same stage. It was deduced that duck Mef2bnb may have the function enhancing fiberⅡformation, besides, the high expression in heart implied that Mef2bnb might relate tothe process of heart development. Therefore,the results of this work may lay the foundation for further study on the structure and the role of Mef2bnb gene in muscle tissues in duck.

关 键 词：鸭 Mef2bnb 克隆 序列分析 发育性表达 

分 类 号：S834[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]