复方参芩对犬细小病毒致心肌组织Bcl-2和Bax mRNA的影响  

Effect of Shenqin Compound on mRNA Expression of Bcl-2 and Bax Genes in Canine Myocardium Infected by Canine Parvovirus

作　　者：刘俊玮[1] 刘娟[1,2] 杜林林[1] 郭志兴[1] 凌榕镔[1] 

机构地区：[1]西南大学荣昌校区动物医学系中药研究开发实验室,荣昌402460 [2]重庆市药效评价工程技术研究中心,重庆402460

出　　处：《畜牧兽医学报》2013年第1期122-128,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目(31172357)

摘　　要：为了探讨复方参芩对犬细小病毒致犬心肌组织Bcl-2和Bax基因表达的影响,将丹参、黄芩、甘草等中药配伍并制备成为复方参芩针剂,人工感染犬细小病毒建立模型;将犬分为空白对照组、模型组、黄芪多糖组、复方参芩组;给药7d后,接种病毒,观察各组犬临床症状,取心肌组织,电镜观察心脏组织超微结构变化,采用荧光实时定量PCR法检测Bcl-2和Bax mRNA表达。结果表明,模型组犬死亡率高,心肌组织结构损伤严重,与空白对照组比较,心肌组织细胞Bcl-2mRNA表达下调(P<0.05),Bax mRNA表达增加(P<0.01)。复方参芩组犬存活率较高,心肌组织损伤轻,与模型组相比,心肌组织细胞Bax mRNA表达下调(P<0.01),Bcl-2mRNA表达上调(P<0.01)。通过本试验证明复方参芩可通过上调犬心肌组织细胞Bcl-2mRNA表达,下调Bax mRNA的表达,抑制细小病毒引起的心肌细胞凋亡,保护犬心肌组织免受细小病毒损害。To investigate the effect of Shenqin compound to Bcl-2 and Bax genes in canine myo- cardium infected by canine parvovirus. Compatibility of salvia, scutellaria, glycyrrhiza and other Chinese herbal to prepare Shenqin compound injection, and to build model by artificial infection of canine parvovirus, the canines were divided into blank control group, model group, astragalus polysaccharide group and Shenqin compound group, after injecting for 7 days, inoculated canine parvovirus, observed clinical symptom in each group, the change of ultrastructure in myocardium was observed through electron microscope. The RT-PCR method were used to test the expression of Bcl-2 and Bax mRNA. The results showed： the model group had high mortality and the myo- cardium was seriously damaged, compared with blank control group, the expression of Bcl-2 mRNA was down-regulated at P〈0.05 level, and the expression of Bax mRNA was up-regulated at P〈0.01 level. In Shenqin compound group, the protective rates were high and myocardium was slightly injured, compared with model group, the expression of Bax mRNA was down-regu- lated at P〈0.01 level, and the expression of Bcl-2 mRNA was up-regulated at P〈0.01 level.This test suggested that Shenqin compound could up-regulated the expression of Bcl-2 mRNA and down-regulated the Bax mRNA to inhibit the myocardial apoptosis and protect canine myocardium from canine parvovirus damage.

关 键 词：犬细小病毒 复方参芩 BCL-2 BAX 荧光定量PCR 

分 类 号：S852.4[农业科学—基础兽医学]