TOLL样受体4对被动致敏人气道平滑肌细胞增殖及合成分泌TGF-β1功能的影响  

Effects of TOLL-like receptor 4 on passively sensitized human airway smoothmuscle cells proliferation and synthesis and secretion function of TGF-β1

作　　者：黄剑伟[1] 莫碧文[1] 韦江红[1] 王昌明[1] 曾锦荣[1] 徐青[1] 

机构地区：[1]广西桂林医学院附属医院呼吸内科,桂林541001

出　　处：《中国应用生理学杂志》2013年第1期20-24,I0001,共6页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金项目(30760083;81260009);广西自然科学基金重点课题(2012GXNSFDA053020);广西科学研究与技术开发计划课题(桂科攻0719006-2-8);广西卫生厅基金(重200732;重200976);广西青年科学基金(桂科青0991085)

摘　　要：目的:探讨TLR4的激活对哮喘血清被动致敏人气道平滑肌细胞(HASMCs)增殖与合成分泌功能的影响。方法:通过培养原代HASMCs,以人哮喘血清致敏细胞,观察HASMCs表面TLR4的表达、细胞增殖反应与转化生子因子-β1(TGF-β1)在哮喘状态下的合成分泌水平。结果:与正常对照组相比,被动致敏组和TNF-α组HASMCsTLR4表达均显著增多(P<0.01),增殖反应显著增强(P<0.01),总蛋白浓度、IgE与TGF-β1的分泌水平均显著增高(P<0.01),并且TNF-α组各项指标均较被动致敏组效果更加显著;抗TLR4抗体组各项指标较被动致敏组、TNF-α组显著减弱或减少(P<0.01)。结论:哮喘血清被动致敏HASMCs表面TLR4的活化可能通过诱导细胞的增殖和TGF-β1的合成分泌,导致哮喘气道微环境的改变,参与哮喘气道重构过程。Objective： To investigate the activation of Toll like receptor 4（TLR4） on passively sensitized human airway smooth muscle cells（HASMCs） proliferation and the synthesis and secretion function.Methods： Through the cultivation of primary HASMCs,we studied TLR4 expression on cell surface,cell proliferation and transformation of parturient factor-β1（TGF-β1） in asthma under the condition of synthesis and secretion level by passively sensitized HASMCs with asthma serum.Results： Compared with the control group,in passive sensitized group and TNF-α group TLR4 expression were significantly increased（P0.01）,significantly enhanced proliferation（P0.01）,total protein concentration,IgE secretion and TGF-β1 were significantly higher（P0.01）;and all the above parameters were increased more significantly in TNF group compared with those in the target effect of passively group;and those parameters were significantly reduced in anti-TLR4 antibody group compared with those in the target effect both of passively sensitized group and TNF-α group.Conclusion： TLR4 on passively sensitized HASMCs activated can induce the excessive proliferation of HASMCs and a large number of synthesis and secretion of TGF-β1,resulting in changing airway micro-environ-ment,which involved in airway remodeling in asthma.

关 键 词：支气管哮喘 TOLL样受体4 人气道平滑肌细胞 被动致敏 分泌 转化生长因子β1 气道重构 

分 类 号：R33[医药卫生—人体生理学]