淫羊藿苷对链脲佐菌素致糖尿病大鼠附睾功能的干预作用  

Amelioration of Icariin for the epididymis impairment induced by streptozocin (STZ) in rats

作　　者：宫燕[1] 史杰[1] 谢高宇[2] 刘浩然[3] 齐敏友[1] 

机构地区：[1]浙江工业大学药学院,杭州310014 [2]贵阳中医学院基础医学院解剖教研室,贵州贵阳550002 [3]湖南大学化学化工学院药理教研室,长沙410082

出　　处：《中国应用生理学杂志》2013年第1期47-50,共4页Chinese Journal of Applied Physiology

基　　金：浙江省教育厅重点课题(Z201121669);湖南大学"青年教师成长计划";湖南大学"中央高校基本科研业务费青年教师科技创新扶持基金(124);黔科合中药字(2012LKZ7027号)

摘　　要：目的:研究淫羊藿苷(Icariin)对链脲佐菌素(STZ)致糖尿病雄性大鼠附睾损伤的影响。方法:SD大鼠给予STZ(60mg/kg ip)建立糖尿病模型。动物随机分为6组(n=14):正常组、模型组、淫羊藿苷低、中、高剂量组(剂量分别为20 mg/kg、40 mg/kg、80 mg/kg)、罗格列酮组(3 mg/kg)。12周后,处死大鼠。检测血清中葡萄糖、附睾中乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)、谷氨酰转肽酶(γ-GT)、α-糖苷酶(α-glucosidase)活力及唾液酸(SA)、果糖(fructose)的含量;取附睾以4%多聚甲醛固定,HE染色,于光镜下观察组织学变化。结果:与正常组比较,糖尿病模型组附睾中LDH、ACP及γ-GT活力降低,唾液酸、果糖含量降低。病理学检测可见附睾管中成熟精子数目减少。淫羊藿苷组比模型组大鼠特异性酶类活力提高,并在组织学上有明显的改善。结论:淫羊藿苷对STZ致雄性大鼠附睾功能损伤有明显的改善作用,其机制可能是增强了附睾相关标志酶的活性及改善了内能量代谢。Objective： To investigate the effects of icariin on the streptozocin（STZ）-induced epididymis impair in rats.Methods： The epididymis impair was induced by injection of streptozocin at dosage of 60 mg/kg ip in SD rats.Animals were randomly divided into six groups（n=14）： normal control,model group,three icariin treated group with different dosages（P.O,20 mg/kg,40 mg/kg,80 mg/kg especially） and rosiglitazon group（P.O,3 mg/kg）,12 weeks later,animals were sacrificed.The level of serum glucose,the activity of lactate dehydrogenase （LDH）,acid phosphatase（ACP）,γ-glutamyltranspeptidase（γ-GT）,α-glucosidase activity as well as sialic acid（SA）,fructose level in the epididymis were determined.The pathological examination was performed under microscope after the epididymis was fixed by 4%polyformalin and stained by HE.Results： Compared with the normal control,the activity of LDH,ACP,γ-GT and α-glucosidase in the epididymis revealed a decline,with lower level of SA and fructose.Histological examination showed that mature spermatocytes in the epididymis markedly decreased.These alterations were ameliorated in the groups with the treatment of icariin at 40 mg/kg and 80 mg/kg,but not at 20mg/kg.Conclusion： Icariin ameliorated the signs of epididymis in diabetic rats induced by streptozocin,this effect might carry out by promoting the elevation of sepicial enzyme and energy metabolism in the epididymis.

关 键 词：淫羊藿苷 链脲佐菌素 附睾 糖尿病 

分 类 号：R285.5[医药卫生—中药学]