GDNF在慢性应激和老化致小鼠行为与认知损伤中的作用  

Role of GDNF in the behavior and cognitive impairment of mice induced by chronic stress and aging

作　　者：李亚[1] 张亚楠[1] 陈亚静[1] 张冠雄[1] 史建勋[1] 

机构地区：[1]曲阜师范大学生命科学学院,山东曲阜273165

出　　处：《中国应用生理学杂志》2013年第1期52-56,共5页Chinese Journal of Applied Physiology

基　　金：山东省自然科学基金资助项目(ZR2009DL009);山东省"十二五"重点学科建设项目

摘　　要：目的:探讨慢性应激对不同月龄小鼠空间学习记忆功能的影响,以及小鼠前脑皮层和海马胶质细胞源性神经营养因子(GDNF)的作用。方法:采用多因素慢性应激动物模型,通过旷场试验和Morris水迷宫试验,检测不同月龄小鼠行为及空间学习记忆能力,并检测GDNF在小鼠脑海马和前脑皮层的表达。结果:与青年(2月龄)小鼠比较,老年(15月龄)小鼠的自发活动和探究行为明显减少,空间学习记忆能力明显降低(P<0.05,P<0.01),且海马CA3区、齿状回和前脑皮层GDNF表达明显下降(P<0.05,P<0.01);在慢性应激后,与对照组比较,青年和老年应激组小鼠的自发活动和探究行为显著减少,空间学习记忆能力显著降低(P<0.05,P<0.01),且小鼠前脑皮层和海马GDNF表达显著下降(P<0.05,P<0.01),老年应激小鼠变化更加显著。结论:脑的老化和慢性应激导致小鼠行为及空间学习记忆功能改变,可能与海马和前脑皮层神经元GDNF表达的变化密切相关。Objective： To investigate the effects of chronic stress on the spatial learning-memory and the role of glial cell line-derived neurotrophic factor （GDNF） of prefrontal cortex（PFC） and hippocampus（HP） in different age mice.Methods： The chronic stress model mice in 21 days with multiple chronic unpredictable stressors were applied.The spontaneous behavior and spatial learning-memory ability of mice were tested,using Open field and Morris water maze task,and the expression of GDNF in HP and PFC were detected by immunohistochemical method. Results： Compared with young mice,the spontaneous behaviors were significantly decreased and the spatial learning-memory function were significantly decreased（P0.05,P0.01） in aged mice.The GDNF expression in the CA3,DG of HP and PFC were significantly reduced in aged mice（P0.05,P0.01）.After chronic stress, the spontaneous behaviors were remarkably decreased and the ability of spatial learning-memory of the stress group mice were significantly decreased（P0.05,P0.01） compared with those of the control group mice.The expression of GDNF in HP and PFC were remarkably reduced（P0.05,P0.01） in stress group mice.The aged stress mice had more serious changes after chronic stress.Conclusion： The brain aging and chronic stress in mice causes behavioral changes and the damage of spatial learning-memory function,and which may be nearly related to the expression of GDNF in HP and PFC.

关 键 词：慢性应激 空间学习记忆 胶质细胞源性神经营养因子 海马 前脑皮层 

分 类 号：Q427[生物学—神经生物学]