HO/CO在甲醛炎性痛大鼠脊髓痛传导及痛觉过敏产生中的作用  

The role of HO/CO in the spinal nociception transmission and hyperalgesia of rats induced by formalin

作　　者：李慧娜[1] 郭莉华[2] 李清君[3] 刘蕾 

机构地区：[1]石家庄市第六医院病理科,石家庄050000 [2]邢台医学高等专科学校,邢台054000 [3]河北医科大学基础医学院病理生理学教研室,石家庄050017 [4]石家庄高等医学专科学校,石家庄050071

出　　处：《中国应用生理学杂志》2013年第1期58-62,共5页Chinese Journal of Applied Physiology

基　　金：河北省科技攻关课题(09276101D-42)

摘　　要：目的:探讨血红素氧合酶/一氧化碳(HO/CO)在甲醛诱导的大鼠自发痛和痛觉过敏形成中的作用。方法:采用鞘内注射的方法,在甲醛炎性痛大鼠和正常大鼠分别给予HO抑制剂Znpp和HO激动剂Hemin;采用加权积分法对痛反应进行评分以代表痛反应程度;采用观察热辐射缩足潜伏期和机械刺激缩足反射阈值表示热和机械性痛觉过敏的程度。结果:Znpp各剂量组与单纯甲醛组相比,大鼠痛反应评分明显降低,且Znpp剂量越大,对大鼠痛反应的抑制作用越明显;与单纯甲醛组相比,Znpp各剂量组大鼠注射足热辐射缩足潜伏期和机械刺激缩足反射阈值均无明显变化,而非注射足热辐射缩足潜伏期和机械刺激缩足反射阈值均明显升高,且Znpp的剂量越大,这种改变越明显。正常大鼠鞘内注射HO的激动剂Hemin后,双侧足热辐射缩足潜伏期和机械刺激缩足反射阈值均明显降低。结论:鞘内给予HO抑制剂可明显抑制甲醛诱导的自发痛反应及热和机械性痛觉过敏程度;正常大鼠鞘内给予HO激动剂可诱发热和机械性痛觉过敏的产生,提示HO/CO系统参与脊髓伤害性信息的传导和痛觉过敏的形成过程。Objective： To investigate the role of heme oxygenase and carbon monoxide（HO/CO） in the development of spontaneous pain and hyperalgesia of rats induced by formalin injection.Methods： Zinc protoporphyrin Znpp（the inhibitor of HO） was intrathecally injected to the rats with formalin inflammatory pain.Hemin（the angonist of HO） was intrathecally injected to the normal rats.The weighted pain scores were used to evaluate the degree of pain response.Thermal withdrawal latency and mechanical withdrawal threshold were observed to assess the degree of thermal hyperalgia and mechanical allodynia.Results： After the intrathecal injection of Znpp,the weighted pain score obviously reduced in a dose-dependent manner compared with the rats with formalin inflammatory pain.Intrathecal injection of Znpp had no obvious effect on thermal withdrawal latency and mechanical withdrawal threshold in injected feet compared with formalin group.But there was a prolongation in a dose-dependent manner in non injected feet.Intrathecal injection of Hemin to normal rats could shorten the thermal withdrawal latency and reduce the mechanical withdrawal threshold on both sides of hindpaws.Conclusion： Intrathecal injection of the HO inhibitor produced prominent inhibition to pain related behavior and thermal and mechanical hyperalgesia induced by formalin injection.Intrathecal injection of HO inductor could induce thermal and mechanical hyperalgesia in normal rats.The results indicated that HO/CO took part in the processes of spinal cord nociceptive information transmission and the development of thermal and mechanical hyperalgesia.

关 键 词：甲醛炎性痛 血红素氧合酶 锌原卟啉 氯化高铁血红素 痛觉过敏 

分 类 号：R338.3[医药卫生—人体生理学]