离心运动对大鼠骨骼肌细胞凋亡和增殖的影响  

The effects of eccentric exercise on the skeletal muscle apoptosis and proliferation in rats

作　　者：宋卫红[1] 汤长发[2] 刘文锋[2] 

机构地区：[1]湖南人文科技学院体育科学系,娄底417000 [2]湖南师范大学体育学院,长沙410012

出　　处：《中国应用生理学杂志》2013年第1期86-90,共5页Chinese Journal of Applied Physiology

基　　金：湖南省教育厅课题资助项目(09C555);湖南省自然科学基金项目(10JJ6041;11JJ6082)

摘　　要：目的:探讨离心运动对骨骼肌细胞凋亡和增殖的时序性影响。方法:50只8周龄SD大鼠随机分为对照组(C)和运动组(B1,B2,B3,B4)(n=10),运动组进行重复3 d的力竭性离心运动,TUNNEL法检测大鼠肱三头肌内侧头不同恢复时相细胞凋亡情况和免疫组化检测其细胞增殖核抗原(PCNA)的表达。结果:①骨骼肌细胞凋亡出现时序性变化,并与运动性骨骼肌微损伤出现了一致性,运动组凋亡指数明显高于对照组(P<0.05),运动后即刻凋亡指数升高,运动后24 h达到峰值,运动后48 h凋亡指数有所下降。②骨骼肌细胞增殖出现时序性,运动组增殖指数明显高于对照组(P<0.05),运动后即刻增殖指数较高,运动后3 h增殖指数有所下降,运动后24 h增殖指数达到峰值,到运动后48 h下降,但还未恢复到对照组。并与细胞凋亡呈中度相关(P<0.05)。结论:①细胞凋亡是诱发骨骼肌再发性损伤的一个因素。②细胞凋亡可能是骨骼肌细胞再生的一个启动因素。Objective： To reveal the effects of three days’ repeated exhausted eccentric exercise on the skeletal muscle apoptosis and proliferation in rats.Methods： Fifty male SD rats aged at 8 week old were randomly divided into control group（C） and training groups（B1,B2,B3,B4）（n=10）,the training groups ran on a treadmill every day till exhausted.After they had been trained repeatedly for three days,their medial head of triceps brachii muscle cell apoptosis was detected in paraffin section by the TUNEL,expression of proliferating cell nuclear antigen（PCNA） protein was examined by immunohistochemistry.Results： ①The apoptosis appeared sequential change,and it was consistent with the exercise-induced skeletal muscle micro-injury（EIMmI）.The apoptosis index in the training group after exercise was much greater than that in the control group（P0.05）,and it reached the peak at 24 h after exercise,then it reduced at 48 h after exercise.②The express of PCNA exhibited a sequential change after exercise,the proliferation index in the training group after exercise was greater than that in the control group（P0.05）,it increased after exercise immediately,but it reduced at 3 h after exercise,then was reached the peak at 24 h after exercise,the proliferation index was moderately correlated with the apoptosis index（P0.05）.Conclusion： ①Cell apoptosis can induce the delayed skeletal muscle damage.② Apoptosis may be a start factor of skeletal muscle regeneration.

关 键 词：重复离心运动 大鼠 运动性骨骼肌微损伤 细胞凋亡 增殖 

分 类 号：G804.2[文化科学—运动人体科学]