分离自陕西泾阳番茄的番茄黄化曲叶病毒(TYLCD)的分子特征  

Molecular Characterization of Tomato yellow leaf curl virus(TYLCD) Infecting Tomato(Lycopersicon esculentum) in Jingyang County of Shaanxi Province

作　　者：阮涛[1] 杨会房[1] 杨水英[1] 孙现超[1] 青玲[1] 

机构地区：[1]西南大学植物保护学院,植物病害生物学重庆市高校级重点实验室,重庆400716

出　　处：《农业生物技术学报》2013年第1期97-105,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.30971897)

摘　　要：从陕西泾阳地区的番茄(Lycopersicon esculentum)上采集到73份表现矮化、黄化和曲叶症状的番茄黄化曲叶病(Tomato yellow leaf curl disease,TYLCD)样品,利用双生病毒简并引物PA和PB及番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)的通用引物TYT-F和TYT-R进行PCR扩增,共有70个样品检测呈阳性;利用双生病毒DNA-B及卫星DNA的通用引物,未扩增到相应目标条带;随机选取样品SX8,利用滚环扩增PCR(RCA-PCR)、克隆及测序等技术获得病毒DNA-A的全基因组序列,该DNA分子全长含2781bp(GenBank登录号:JN412854),与来自山东番茄的TYLCV分离物SD2(缩写为TYLCV-[SD2])(GenBank登录号:GU199587)的核苷酸序列相似性最高,为99.9%;系统进化分析发现,该病毒分离物与我国已报道的TYLCV各分离物亲缘关系较近。这些结果表明,陕西番茄黄化曲叶病是由TYLCV引起,该病毒可能来源于我国山东番茄上的TYLCV。A total of 73 tomato （Lycopersicon esculentum） samples exhibiting stunting, yellowing and leaf-curling symptoms of tomato yellow leaf curl disease （TYLCD） were collected in Jingyang, Shaanxi Province. Using degenerate primer pair PA and PB of geminiviruses and specific primer pair TYT-F and TYT-R of Tomato yellow leaf curl virus （TYLCV）, geminiviruses and TYLCV were detected in 70 samples by PCR. The DNA-B and satellite DNA molecules of geminiviruses were not detectable from all these samples with their universal primers respectively. Using rolling circle amplification PCR （RCA-PCR）, cloning and sequencing techniques, the complete nucleotide sequence of isolate SX8 （GenBank Accession No. JN412854） was determined to be 2 781 bp and has highly identity of nucleotide sequences （99.9%） with TYLCV-[SD2] （GenBank Accession No. GU199587）isolated from tomato in Shandong Province. The phylogenetic analysis indicated that TYLCV-[SXS] shared high relativity with those previously reported isolates of TYLCV in China.These results suggest that TYLCD in Shaanxi Province is caused by TYLCV which may originate from DNA-A molecules of TYLCV infecting tomato in Shandong Province.

关 键 词：番茄 陕西泾阳 番茄黄化曲叶病 番茄黄化曲叶病毒 分子特征 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]